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SDS-Page

Lipocalin 1 Recombinant Protein | LCN1 recombinant protein

Recombinant Lipocalin 1 (LCN1)

Gene Names
LCN1; TP; TLC; PMFA; VEGP
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Lipocalin 1; Recombinant Lipocalin 1 (LCN1); LCN1 recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 350ug/mL (varies by lot)
Sequence
PTTEENQDV SGTWYLKAAA WDKEIPDKKF GSVSVTPMKI KTLEGGNLQV KFTVLIAGRC KEMSTVLEKT DEPAKYTAYS GKQVLYIIPS SVEDHYIFYY EGKIHRHHFQ IAKLVGRDPE INQEALEDFQ SVVRAGGLNPDNIFIPKQSE TCP
Applicable Applications for LCN1 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Organism Species
Rattus norvegicus (Rat)
Source
Prokaryotic expression
Residues
Pro22~Pro173
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
6.6
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 21.0kDa
Accurate Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
lipocalin-1 isoform 1
NCBI Official Synonym Full Names
lipocalin 1
NCBI Official Symbol
LCN1
NCBI Official Synonym Symbols
TP; TLC; PMFA; VEGP
NCBI Protein Information
lipocalin-1
UniProt Protein Name
Lipocalin-1
Protein Family
UniProt Gene Name
LCN1
UniProt Synonym Gene Names
VEGP; Tlc; TP; VEG protein
UniProt Entry Name
LCN1_HUMAN

NCBI Description

This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9. [provided by RefSeq, Nov 2011]

Uniprot Description

LCN1: Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; extracellular region

Molecular Function: cysteine protease inhibitor activity

Biological Process: long-chain fatty acid transport; retinal homeostasis; sensory perception of taste; response to stimulus; proteolysis; transmembrane transport

Research Articles on LCN1

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Product Notes

The LCN1 lcn1 (Catalog #AAA2029647) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lipocalin 1 can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). Researchers should empirically determine the suitability of the LCN1 lcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PTTEENQDV SGTWYLKAAA WDKEIPDKKF GSVSVTPMKI KTLEGGNLQV KFTVLIAGRC KEMSTVLEKT DEPAKYTAYS GKQVLYIIPS SVEDHYIFYY EGKIHRHHFQ IAKLVGRDPE INQEALEDFQ SVVRAGGLNP DNIFIPKQSE TCP. It is sometimes possible for the material contained within the vial of "Lipocalin 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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