EphB4 active protein

EphB4 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122842

• Gene Names: Ephb4; Htk; MDK2; Myk1; Tyro11; AI042935; b2b2412Clo
• Purity: >97% as determined by SDS-PAGE
• Synonyms: EphB4; EphB4 Protein; Mouse; Recombinant (hFc Tag); Mouse EphB4/HTK Protein (Fc Tag); AI042935 Protein; Htk Protein; MDK2 Protein; Myk1 Protein; Tyro11 Protein; EPH receptor B4; EphB4 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala539

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 16
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse EPHB4 (P54761-1) extracellular domain (Met 1-Ala 539) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Ephrin B2 His at 2ug/ml (100uL/well) can bind Mouse EphB4 hFc. The EC50 of Mouse EphB4 hFc is 2.0-10.0ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized Mouse Ephrin B2 His at 2 ug/ml (100 ul/well) can bind Mouse EphB4 hFc, the EC50 of Mouse EphB4 hFc is 2.0-10.0 ng/mL.)

Related Product Information for EphB4 active protein

Background: Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Product Categories/Family for EphB4 active protein

Actin Dynamics Signaling Pathway

References

Davalos V, et al. (2006) EPHB4 and survival of colorectal cancer patients. Cancer Res. 66(18): 8943-8.Zhao C, et al. (2006) Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab. 4(2): 111-21.Kertesz N, et al. (2006) The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth. Blood. 107(6): 2330-8.Noren NK, et al. (2007) Paradoxes of the EphB4 receptor in cancer. Cancer Res. 67(9): 3994-7.Taylor AC, et al. (2007) EphB4 expression along adult rat microvascular networks: EphB4 is more than a venous specific marker. Microcirculation. 14(3): 253-67.

NCBI and Uniprot Product Information

• NCBI GI #: 227330573
• NCBI GeneID: 13846
• NCBI Accession #: NP_034274.4
• NCBI GenBank Nucleotide #: NM_010144.6
• UniProt Accession #: P54761; Q60627; Q99MR2
• Molecular Weight: 108,848 Da
• NCBI Official Full Name: ephrin type-B receptor 4 isoform b
• NCBI Official Synonym Full Names: Eph receptor B4
• NCBI Official Symbol: Ephb4
• NCBI Official Synonym Symbols: Htk; MDK2; Myk1; Tyro11; AI042935; b2b2412Clo
• NCBI Protein Information: ephrin type-B receptor 4
• UniProt Protein Name: Ephrin type-B receptor 4
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: Ephb4
• UniProt Synonym Gene Names: Htk; Mdk2; Myk1; mDK-2

Uniprot Description

EphB4: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 5|5 G2

Cellular Component: integral to plasma membrane

Molecular Function: ephrin receptor activity; protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: angiogenesis; cell adhesion; cell migration during sprouting angiogenesis; ephrin receptor signaling pathway; heart morphogenesis; protein amino acid autophosphorylation; venous blood vessel morphogenesis