Human endothelial PAS domain protein 1 ELISA Kit | EPAS1 elisa kit

Human hypoxia-inducible factor 2, HIF-2 ELISA Kit

Cat. Num. AAA702348

• Gene Names: EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
• Reactivity: Human
• Synonyms: endothelial PAS domain protein 1; Human hypoxia-inducible factor 2; HIF-2 ELISA Kit; Human hypoxia-inducible factor 2 (HIF-2) ELISA kit; ECYT4; HIF2A; HLF; MOP2; PASD2; bHLHe73; HIF-1alpha-like factor; basic-helix-loop-helix-PAS protein MOP2; hypoxia-inducible factor 2 alpha; member of PAS protein 2; EPAS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human HIF-2. No significant cross-reactivity or interference between human HIF-2 and analogues was observed.
• Sequence Length: 870

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: < 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPAS1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIF-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 40254439
• NCBI GeneID: 2034
• NCBI Accession #: NP_001421.2
• NCBI GenBank Nucleotide #: NM_001430.4
• UniProt Accession #: Q99814; Q86VA2; Q99630
• Molecular Weight: 96,459 Da
• NCBI Official Full Name: endothelial PAS domain-containing protein 1
• NCBI Official Synonym Full Names: endothelial PAS domain protein 1
• NCBI Official Symbol: EPAS1
• NCBI Official Synonym Symbols: HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
• NCBI Protein Information: endothelial PAS domain-containing protein 1; EPAS-1; HIF2-alpha; HIF-2-alpha; HIF-1alpha-like factor; HIF-1-alpha-like factor; member of PAS protein 2; PAS domain-containing protein 2; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; basic-helix-loop-helix-PAS protein MOP2; class E basic helix-loop-helix protein 73
• UniProt Protein Name: Endothelial PAS domain-containing protein 1
• Protein Family: Endothelial PAS domain-containing protein
• UniProt Gene Name: EPAS1
• UniProt Synonym Gene Names: BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha
• UniProt Entry Name: EPAS1_HUMAN

NCBI Description

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

Uniprot Description

Function: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.