Glutathione Peroxidase, Western Blot Control Enzyme | RB2244 enzyme
Glutathione Peroxidase, Human, Western Blot Control
Applications
Western Blot
Purity
Serum
Synonyms
Glutathione Peroxidase; Western Blot Control; Human; RB2244 enzyme
Purity/Purification
Serum
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Applicable Applications for RB2244 enzyme
Western Blot (WB)
Application Notes
Suitable for use in Western Blot as a control. Load 10ul/lane for good visibility with antibody
Note
SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2X sample buffer per 10ul of the solution prior to heating and loading on gels. This preparation is not biologically active. It is not suitable for ELISA or other applications where native protein is required. This preparation is intended for qualitative purpose and not to serve as standard of known concentration.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for RB2244 enzyme
Highly reactive and potentially dangerous reactive oxygen species (ROS) are normally produced within the cells, primarily from the mitochondrial respiratory chain where in excess electrons are donated to molecular oxygen (o2) to generate peroxide anion (O2-). Superoxide anion is reduced by the superoxide dismutase (SOD) to hydrogen peroxide (H2O2) and hydrogen peroxide is reduced to water (H2O) by catalase, located primarily in the peroxisomes, and by glutathione peroxidase (GPx), located in the mitochondria and cytosol. Hydrogen peroxide, in the presence of transitions metals, can be converted to the highly toxic hydroxyl radical (OH.) and all three of the ROS (O2-, H2O2, and OH.) can damage macromolecules (proteins, DNA etc). The GPxs are commonly considered the most important for ROS defense since they have broader substrate specificities and stronger affinity for H2O2 than catalases.
Product Categories/Family for RB2244 enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
glutathione peroxidase
NCBI Official Symbol
RB2244
NCBI Protein Information
glutathione peroxidase
Similar Products
Product Notes
The RB2244 (Catalog #AAA653925) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glutathione Peroxidase, Western Blot Control can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot as a control. Load 10ul/lane for good visibility with antibody. Researchers should empirically determine the suitability of the RB2244 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione Peroxidase, Western Blot Control, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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