Eosinophil Peroxidase Enzyme | EPX enzyme

Eosinophil Peroxidase, Human (EPO, EPX, EPER, EPP)

Cat. Num. AAA653827

• Gene Names: EPX; EPO; EPP; EPX-PEN
• Purity: Highly Purified; ~95% (SDS-PAGE)
• Synonyms: Eosinophil Peroxidase; Human (EPO; EPX; EPER; EPP); EPX enzyme

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a lyophillized powder from 20mM NaH2PO4, pH 7.5, 150mM sodium chloride. Reconstitute with deionized dH2O.

• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

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Related Product Information for EPX enzyme

Native, human eosinophil peroxidase is a secreted hemoprotein that catalyzes oxidations of various substrates by hydrogen peroxide. Together with other leukocyte-derived enzymes, such as Myeloperoxidase, EPO plays an important role in the pathogenesis of asthma by mediating oxidative events that lead to the production of ROS (reactive oxygen species) and RNS (reactive nitrogen species).

Product Categories/Family for EPX enzyme

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 182146
• NCBI GeneID: 8288
• UniProt Accession #: P11678; Q4TVP3
• Molecular Weight: ~70.5kD
• NCBI Official Full Name: eosinophil peroxidase
• NCBI Official Synonym Full Names: eosinophil peroxidase
• NCBI Official Symbol: EPX
• NCBI Official Synonym Symbols: EPO; EPP; EPX-PEN
• NCBI Protein Information: eosinophil peroxidase
• UniProt Protein Name: Eosinophil peroxidase
• Protein Family: Eosinophil peroxidase
• UniProt Gene Name: EPX
• UniProt Synonym Gene Names: EPER; EPO; EPP; EPO
• UniProt Entry Name: PERE_HUMAN

NCBI Description

This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded precursor protein is processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a cluster of three peroxidase genes at chromosome 17q23. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Ref.5 Ref.6

Catalytic activity: 2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactor: Binds 1 calcium ion per heterodimer

By similarity.Binds 1 heme B (iron-protoporphyrin IX) covalently through ester linkages to hydroxylated methyl groups formed auto-catalytically with hydrogen peroxide at the heme C-1 and C-5 positions. The ester linkage to Asp-232 was observed in 30% of the chains.

Subunit structure: Tetramer of two light chains and two heavy chains.

Subcellular location: Cytoplasmic granule. Note: Cytoplasmic granules of eosinophils.

Polymorphism: Allelic variant in EPX is associated with Japanese cedar pollinosis which is a type I allergic disease with ocular and nasal symptoms that develop paroxysmally on contact with Japanese cedar pollen. These symptoms, which occur seasonally each year, are typical features of allergic rhinitis, such as sneezing, excessive nasal secretion, nasal congestion, and conjunctival itching.

Involvement in disease: Eosinophil peroxidase deficiency (EPD) [MIM:261500]: Autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7

Sequence similarities: Belongs to the peroxidase family. XPO subfamily.