CD39 / ENTPD1 recombinant protein

Recombinant Mouse CD39 / ENTPD1 Protein (His tag)

Cat. Num. AAA2547002

• Gene Names: Entpd1; Cd39; AA408691; NTPDase-1; 2610206B08Rik
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: CD39 / ENTPD1; Recombinant Mouse CD39 / ENTPD1 Protein (His tag); 2610206B08Rik; AA408691; Cd39; NTPDase-1; CD39 / ENTPD1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
• Sequence Length: 510

• Application Notes: The secreted recombinant mouse ENTPD1 consists of 451 amino acids and has a calculated molecular mass of 51 kDa. The recombinant protein migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Thr 38
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD39 / ENTPD1 recombinant protein

Background: CD39, also known as ENTPD1, belongs to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. It is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism. There is a correlation between ATP hydrolysis and triglycerides in patients with chronic heart disease, suggesting a relationship between ATP diphosphohydrolase and thrombogenesis. In the nervous system, CD39 could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.

Description: A DNA sequence encoding the mouse ENTPD1 (P55772) extracellular domain (Thr 38-Ile 478) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6753346
• NCBI GeneID: 12495
• NCBI Accession #: NP_033978.1
• NCBI GenBank Nucleotide #: NM_009848.4
• UniProt Accession #: P55772
• Molecular Weight: 57,205 Da
• NCBI Official Full Name: ectonucleoside triphosphate diphosphohydrolase 1 isoform 2
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 1
• NCBI Official Symbol: Entpd1
• NCBI Official Synonym Symbols: Cd39; AA408691; NTPDase-1; 2610206B08Rik
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 1
• UniProt Protein Name: Ectonucleoside triphosphate diphosphohydrolase 1
• UniProt Gene Name: Entpd1
• UniProt Synonym Gene Names: Cd39; NTPDase 1; Ecto-ATPDase 1; Ecto-ATPase 1

Uniprot Description

ENTPD1: an ectonucleotidase that, like CD73, hydrolyzes immunostimulatory ATP, and ADP, to immunosuppressive AMP. May inhibit host immune responses against cancer. Expressed at high levels in malignant epithelial cells of human rectal adenocarcinoma. A prognostic marker of chronic lymphocytic leukemia that may identify patients with an unfavorable clinical outcome. Synergizes with CD161 to modulate Th17 responses in Crohn's disease. It may hydrolyze ATP and other nucleotides in the nervous system to regulate purinergic neurotransmission in the nervous system. May be implicated in inhibiting platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Belongs to the GDA1/CD39 NTPase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.1.5; Extracellular matrix; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 19 C3|19 34.25 cM

Cellular Component: basal lamina; basolateral plasma membrane; cell soma; cell surface; extracellular space; membrane; neuron projection; postsynaptic density; synaptic vesicle

Molecular Function: adenosine-diphosphatase activity; ATPase activity; identical protein binding; nucleoside-diphosphatase activity; nucleoside-triphosphatase activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of dopamine secretion; platelet activation; protein homooligomerization; purine ribonucleoside diphosphate catabolic process