ENSA recombinant protein

ENSA Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121668

• Gene Names: ENSA; ARPP-19e
• Purity: >90% as determined by SDS-PAGE
• Synonyms: ENSA; ENSA Protein; Human; Recombinant (His Tag); Human ENSA/Endosulfine alpha Protein (His Tag); ARPP-19e Protein; endosulfine alpha; ENSA recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu121

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human ENSA (O43768-1)(Met1-Glu121) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ENSA recombinant protein

Background: Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

References

Ye M. et al., 2001, Genome Res. 10 (10): 1546-60. Apiou F. et al., 1999, Diabetes 48 (9): 1873-6. Lennon G. et al., 1997, Genome Res. 6 (9): 791-806.

NCBI and Uniprot Product Information

• NCBI GI #: 4758272
• NCBI GeneID: 2029
• NCBI Accession #: NP_004427.1
• NCBI GenBank Nucleotide #: NM_004436.2
• UniProt Accession #: O43768; Q5T5H2; Q68D48; Q6FHW0; Q6IAM4; Q6NUL2; Q6VUC6; Q6VUC7; Q6VUC8; Q6VUC9; A8K1Z9; E9PB69
• Molecular Weight: 14,868 Da
• NCBI Official Full Name: alpha-endosulfine isoform 3
• NCBI Official Synonym Full Names: endosulfine alpha
• NCBI Official Symbol: ENSA
• NCBI Official Synonym Symbols: ARPP-19e
• NCBI Protein Information: alpha-endosulfine
• UniProt Protein Name: Alpha-endosulfine
• Protein Family: Alpha-endosulfine
• UniProt Gene Name: ENSA
• UniProt Entry Name: ENSA_HUMAN

NCBI Description

The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

ENSA: a cytoplasmic, highly conserved cAMP-regulated phosphoprotein (ARPP) and regulator of ATP-sensitive potassium (KATP) channels. An endogenous ligand for the sulfonylurea receptor, ABCC8, the regulatory subunit of the KATP channel located on the plasma membrane of pancreatic beta cells. Modulates insulin secretion through the interaction with ABCC8, and may be associated with type 2 diabetes. Eight alternatively spliced isoforms have been observed.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: phosphatase inhibitor activity; protein phosphatase 2A binding; protein phosphatase type 2A regulator activity; protein phosphatase inhibitor activity; potassium channel inhibitor activity; ion channel inhibitor activity; receptor binding

Biological Process: mitosis; regulation of catalytic activity; transport; cell division; negative regulation of catalytic activity; response to glucose stimulus; mitotic cell cycle; G2/M transition of mitotic cell cycle; regulation of insulin secretion; response to nutrient