Human enolase 1, (alpha) ELISA Kit | ENO1 elisa kit

Human Alpha-enolase, ENO1/ENO1L1/MBPB1/MPB1 ELISA Kit

Cat. Num. AAA706020

• Gene Names: ENO1; NNE; PPH; MPB1; ENO1L1
• Reactivity: Human
• Synonyms: enolase 1; (alpha); Human Alpha-enolase; ENO1/ENO1L1/MBPB1/MPB1 ELISA Kit; Human Alpha-enolase (ENO1/ENO1L1/MBPB1/MPB1) ELISA kit; ENO1L1; MBP-1; MPB1; NNE; PPH; 2-phospho-D-glycerate hydro-lyase; MYC promoter-binding protein 1; alpha enolase like 1; non-neural enolase; phosphopyruvate hydratase; tau-crystallin; ENO1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ENO1. No significant cross-reactivity or interference between human ENO1 and analogues was observed.
• Sequence Length: 341

• Samples: Serum, tissue homogenates, plasma
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: < 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ENO1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 319996655
• NCBI GeneID: 2023
• NCBI Accession #: NP_001188412.1
• NCBI GenBank Nucleotide #: NM_001201483.1
• UniProt Accession #: P06733; P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59
• Molecular Weight: 47,169 Da
• NCBI Official Full Name: c-myc promoter-binding protein-1 isoform MBP-1
• NCBI Official Synonym Full Names: enolase 1, (alpha)
• NCBI Official Symbol: ENO1
• NCBI Official Synonym Symbols: NNE; PPH; MPB1; ENO1L1
• NCBI Protein Information: alpha-enolase; c-myc promoter-binding protein-1; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Alpha-enolase
• Protein Family: Enolase
• UniProt Gene Name: ENO1
• UniProt Synonym Gene Names: ENO1L1; MBPB1; MPB1; NNE
• UniProt Entry Name: ENOA_HUMAN

NCBI Description

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Uniprot Description

Function: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.5 PublicationsManual assertion based on experiment in:Ref.3

Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.1 PublicationManual assertion based on experiment in:Ref.18

Cofactor: Magnesium. Binds two Mg2+ per subunit. Required for catalysis and for stabilizing the dimer.1 PublicationManual assertion based on experiment in:Ref.39