Eng recombinant protein

Eng, 27-581aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206367

• Gene Names: Eng; Endo; CD105; AI528660; AI662476; S-endoglin
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Eng; 27-581aa; Mouse; His tag; Baculovirus; Endoglin; AI528660; AI662476; CD105; Endo; S-endoglin; Eng recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ERVGCDLQPV DPTRGEVTFT TSQVSEGCVA QAANAVREVH VLFLDFPGML SHLELTLQAS KQNGTETQEV FLVLVSNKNV FVKFQAPEIP LHLAYDSSLV IFQGQPRVNI TVLPSLTSRK QILDWAATKG AITSIAALDD PQSIVLQLGQ DPKAPFLCLP EAHKDMGATL EWQPRAQTPV QSCRLEGVSG HKEAYILRIL PGSEAGPRTV TVMMELSCTS GDAILILHGP PYVSWFIDIN HSMQILTTGE YSVKIFPGSK VKGVELPDTP QGLIAEARKL NASIVTSFVE LPLVSNVSLR ASSCGGVFQT TPAPVVTTPP KDTCSPVLLM SLIQPKCGNQ VMTLALNKKH VQTLQCTITG LTFWDSSCQA EDTDDHLVLS SAYSSCGMKV TAHVVSNEVI ISFPSGSPPL RKKVQCIDMD SLSFQLGLYL SPHFLQASNT IELGQQAFVQ VSVSPLTSEV TVQLDSCHLD LGPEGDMVEL IQSRTAKGSC VTLLSPSPEG DPRFSFLLRV YMVPTPTAGT LSCNLALRPS TLSQEVYKTV SMRLNIVSPD LSGKGLEHHH HHH
• Sequence Length: 653

• Applicable Applications for Eng recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Eng recombinant protein

Eng, also known as endoglin, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. It is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. Recombinant mouse Eng, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Eng recombinant protein

Immunology

References

Ten Dijke P., et al. (2008) Angiogenesis. 11:79-89.; Bernabeu C., et al. (2007) J Cell Biochem. 102:1375-1388.

NCBI and Uniprot Product Information

• NCBI GI #: 226437647
• NCBI GeneID: 13805
• NCBI Accession #: NP_031958
• NCBI GenBank Nucleotide #: NM_007932.2
• UniProt Accession #: Q63961; Q61520; Q8K100
• Molecular Weight: 60.9kDa (563aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: endoglin isoform 1
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: Eng
• NCBI Official Synonym Symbols: Endo; CD105; AI528660; AI662476; S-endoglin
• NCBI Protein Information: endoglin
• UniProt Protein Name: Endoglin
• Protein Family: Endoglin
• UniProt Gene Name: Eng
• UniProt Synonym Gene Names: Edg

Uniprot Description

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:10625534). Required for normal structure and integrity of adult vasculature (). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (PubMed:10625534). May play a role in the binding of endothelial cells to integrins. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 ().