Human Endoglin ELISA Kit | ENG elisa kit

Human Endoglin ELISA Kit

Cat. Num. AAA763784

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Human
• Synonyms: Endoglin; Human Endoglin ELISA Kit; Endoglin/CD105/ENG/END/HHT1/ORW/ORW1; ENG elisa kit

• Reactivity: Human
• Sequence Length: 658

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ENG elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human Endoglin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Endoglin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Endoglin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Endoglin can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 168693647
• NCBI GeneID: 2022
• NCBI Accession #: NP_001108225.1
• NCBI GenBank Nucleotide #: NM_001114753.2
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: endoglin isoform 1
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin
• UniProt Protein Name: Endoglin
• Protein Family: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END
• UniProt Entry Name: EGLN_HUMAN

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: cell surface; cytoplasm; external side of plasma membrane; extracellular space; focal adhesion; nucleoplasm; receptor complex

Molecular Function: activin binding; galactose binding; glycosaminoglycan binding; protein binding; protein homodimerization activity; punt binding; transforming growth factor beta binding; transforming growth factor beta receptor activity; transforming growth factor beta receptor, cytoplasmic mediator activity; transmembrane receptor activity

Biological Process: artery morphogenesis; BMP signaling pathway; cell adhesion; cell migration; cell motility involved in cell locomotion; central nervous system vasculogenesis; chronological cell aging; extracellular matrix disassembly; heart looping; negative regulation of cell migration; negative regulation of endothelial cell proliferation; negative regulation of nitric-oxide synthase activity; negative regulation of protein amino acid autophosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; patterning of blood vessels; positive regulation of BMP signaling pathway; positive regulation of protein amino acid phosphorylation; positive regulation of systemic arterial blood pressure; positive regulation of transcription from RNA polymerase II promoter; regulation of cell adhesion; regulation of cell proliferation; regulation of phosphorylation; regulation of transcription, DNA-dependent; regulation of transforming growth factor beta receptor signaling pathway; response to hypoxia; smooth muscle development; transforming growth factor beta receptor signaling pathway; vasculogenesis; venous blood vessel morphogenesis; wound healing

Disease: Telangiectasia, Hereditary Hemorrhagic, Of Rendu, Osler, And Weber