Elastin Microfibril Interface Located Protein 1 Recombinant Protein | EMILIN1 recombinant protein

Recombinant Elastin Microfibril Interface Located Protein 1 (EMILIN1)

Cat. Num. AAA2031693

• Gene Names: EMILIN1; EMI; gp115; EMILIN
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Elastin Microfibril Interface Located Protein 1; Recombinant Elastin Microfibril Interface Located Protein 1 (EMILIN1); EMILIN1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.1 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QLSLK DLTGPAGEAG PPGPPGLQGP PGPAGPPGSP GKDGQEGPIG PPGPQGEQGV EGAPAAPVPQ VAFSAALSLP RSEPGTVPFD RVLLNDGGYY DPETGVFTAP LAGRYLLSAV LTGHRHEKVE AVLSRSNQGV ARVDSGGYEP EGLENKPVAE SQPSPGTLGV FSLILPLQAG DTVCVDLVMGQLAHSEEPLT IFSGALLYGD PELEHA
• Sequence Length: 1016

• Applicable Applications for EMILIN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 25.2kDa
• Accurate Molecular Mass (KD): 31kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gln806~Ala1016
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5901944
• NCBI GeneID: 11117
• NCBI Accession #: NP_008977.1
• NCBI GenBank Nucleotide #: NM_007046.3
• Molecular Weight: 35,928 Da
• NCBI Official Full Name: EMILIN-1
• NCBI Official Synonym Full Names: elastin microfibril interfacer 1
• NCBI Official Symbol: EMILIN1
• NCBI Official Synonym Symbols: EMI; gp115; EMILIN
• NCBI Protein Information: EMILIN-1
• UniProt Protein Name: EMILIN-1
• Protein Family: EMILIN
• UniProt Gene Name: EMILIN1
• UniProt Synonym Gene Names: EMI; Elastin microfibril interfacer 1
• UniProt Entry Name: EMIL1_HUMAN

NCBI Description

This gene encodes an extracellular matrix glycoprotein that is characterized by an N-terminal microfibril interface domain, a coiled-coiled alpha-helical domain, a collagenous domain and a C-terminal globular C1q domain. The encoded protein associates with elastic fibers at the interface between elastin and microfibrils and may play a role in the development of elastic tissues including large blood vessels, dermis, heart and lung. [provided by RefSeq, Sep 2009]

Uniprot Description

EMILIN1: May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Cell adhesion; Extracellular matrix

Chromosomal Location of Human Ortholog: 2p23.3-p23.2

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; extracellular region

Molecular Function: identical protein binding; protein binding; extracellular matrix constituent conferring elasticity

Biological Process: extracellular matrix organization and biogenesis; cell adhesion