Mouse anti-Human Topo II alpha Monoclonal Antibody | anti-TOP2A antibody

Topo II alpha

Cat. Num. AAA668217

• Gene Names: TOP2A; TOP2; TP2A
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein G purification
• Synonyms: Topo II alpha; Monoclonal Antibody; anti-TOP2A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, Kappa
• Clone Number: ABM48B4
• Purity/Purification: Protein G purification
• Form/Format: Purified
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1531

• Applicable Applications for anti-TOP2A antibody: Western Blot (WB), Flow Cytometry (FACS).
• Application Notes: Western blot analysis: 1-2 ug/ml; FACS Analysis: 0.5-1 ug/10^6 cells
• Immunogen: A partial length recombinant human Topo II alpha protein (amino acids 1326-1512) was used as the immunogen for this antibody.
• Content: 25 ug in 50 ul/100 ug in 200 ?l PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
• Target Modification: No
• Produced from Ascites: No
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (FACS)

(Intracellular Flow analysis of Topo II alpha antibody in Jurkat cells using 0.5 ug/10^6 cells of anti-Topo II alpha antibody (ABM48B4). Green represents isotype control;red represents anti-Topo II alpha antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

Western Blot (WB)

(Expression analysis of Topo II alpha. Anti-Topo II alpha antibody (Clone: ABM48B4) was tested at 1 ug/ml on (1) HT-29, (2) 293, (3) A549 and (4) Raji Lysates.)

Related Product Information for anti-TOP2A antibody

Topoisomerases are essential nuclear enzymes that are involved in DNA supercoiling regulation and play key roles in transcription, replication, and chromosome segregation. Two major classes of topoisomerases (types I and II) are distinguished by the number of DNA strands that they cleave and the mechanism by which they alter the topological properties of DNA. Topo II Alpha is cell cycle-regulated and is essential for the survival of proliferating cells. It accumulates on chromatin during M-phase, a dynamic localization that is dependent on its C-terminal domain. Although Topo II alpha is the major form of Topo II responsible for decatenation, mitotic chromosome formation and chromosome segregation in proliferating cells, the contribution of the two isoforms has not yet been fully established. Topo II Alpha plays an important role in DNA synthesis and transcription and has been implicated in a variety of human cancers. It also plays a role in various cancer subtypes and acts as targets for cancer therapy, as well as biomarkers for prediction of response.

NCBI and Uniprot Product Information

• NCBI GI #: 19913406
• NCBI GeneID: 7153
• NCBI Accession #: NP_001058.2
• NCBI GenBank Nucleotide #: NM_001067.3
• UniProt Accession #: P11388; Q71UN1; Q71UQ5; Q9HB24; Q9HB25; Q9HB26; Q9UP44; Q9UQP9; B2RTS1
• NCBI Official Full Name: DNA topoisomerase 2-alpha
• NCBI Official Synonym Full Names: topoisomerase (DNA) II alpha
• NCBI Official Symbol: TOP2A
• NCBI Official Synonym Symbols: TOP2; TP2A
• NCBI Protein Information: DNA topoisomerase 2-alpha
• UniProt Protein Name: DNA topoisomerase 2-alpha
• Protein Family: DNA topoisomerase
• UniProt Gene Name: TOP2A
• UniProt Synonym Gene Names: TOP2
• UniProt Entry Name: TOP2A_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Uniprot Description

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation ().