IL-17F elispot set

Human IL-17F ELISpot Pair

Cat. Num. AAA335775

• Gene Names: IL17F; ML1; ML-1; CANDF6; IL-17F
• Synonyms: IL-17F; Human IL-17F ELISpot Pair; IL-17F elispot set

• Specificity: The assay recognizes natural human IL-17F. To define specificity several proteins were tested for cross reactivity. There was no cross reactivity observed for these proteins tested IL-17A, IL-17B, IL-17D, IL17E, IL-5, IL-23, Perforin. The antibody pair shows cross reactivity with the human IL17A/F heterodimer. This testing was performed using the equivalent IL-17F antibody pair in an ELISA assay.
• Sequence Length: 163

• Assay Type: Sandwich

Related Product Information for IL-17F elispot set

Intended Uses: ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, canerology, infectious disease, autoimmune diseases and tranplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Principle of the Assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

NCBI and Uniprot Product Information

• NCBI GI #: 16418375
• NCBI GeneID: 112744
• NCBI Accession #: NP_443104.1
• NCBI GenBank Nucleotide #: NM_052872.3
• Molecular Weight: 18,045 Da
• NCBI Official Full Name: interleukin-17F
• NCBI Official Synonym Full Names: interleukin 17F
• NCBI Official Symbol: IL17F
• NCBI Official Synonym Symbols: ML1; ML-1; CANDF6; IL-17F
• NCBI Protein Information: interleukin-17F
• UniProt Protein Name: Interleukin-17F
• UniProt Gene Name: IL17F
• UniProt Synonym Gene Names: IL-17F
• UniProt Entry Name: IL17F_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17F: Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. Belongs to the IL-17 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity; cytokine binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; protein binding; protein homodimerization activity

Biological Process: cartilage development; cytokine biosynthetic process; inflammatory response; lymphotoxin A biosynthetic process; negative regulation of angiogenesis; regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; regulation of interleukin-2 biosynthetic process; regulation of interleukin-6 biosynthetic process; regulation of interleukin-8 biosynthetic process; regulation of transforming growth factor beta receptor signaling pathway

Disease: Candidiasis, Familial, 6