Testing Data
CLEC4D elisa pair set
CLEC4D Matched ELISA Antibody Pair Set, Human
Synonyms
CLEC4D; CLEC4D Matched ELISA Antibody Pair Set; Human; Human CLEC4D/CLECSF8 ELISA Pair Set; C-type lectin domain family 4; member D; CD368 Matched ELISA Antibody Pair Set; CLEC-6 Matched ELISA Antibody Pair Set; CLEC6 Matched ELISA Antibody Pair Set; CLECSF8 Matched ELISA Antibody Pair Set; Dectin-3 Matched ELISA Antibody Pair Set; MCL Matched ELISA Antibody Pair Set; MPCL Matched ELISA Antibody Pair Set; CLEC4D elisa pair set
Specificity
Quantitative determination of Human CLEC4D
Assay Type
Solid Phase Sandwich ELISA
Detection Range
23.44-1500pg/mL
Sensitivity
24pg/ml
Materials Provided
1. Capture Antibody 0.3 mg/mL of mouse anti-CLEC4D monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL rabbit anti-CLEC4D polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 80 ng of recombinant CLEC4D. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5ng/mL is recommended.
2. Detection Antibody 0.5 mg/mL rabbit anti-CLEC4D polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 80 ng of recombinant CLEC4D. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CLEC4D elisa pair set
C-type lectin (CLEC) family is a type of carbohydrate-binding protein domain named lectin. C-type lectins are the most diverse and prevalent lectin family in immunity with its requirement for calcium for binding. Proteins including a C-type lectin domain have diverse range of functions including cell-cell adhesion, immune response to pathogens and apoptosis. There are at least 14 types of C-type lectins: typeI to typeI©IV. CLEC4D(CLECSF8) is a typeII membrane glycoprotein belonging to the C-type lectin family, with restricted expression in the monocyte/macrophage lineage. It plays important roles in the function of macrophages.
This CLEC4D Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CLEC4D. It contains Human CLEC4D capture antibody, Human CLEC4D detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CLEC4D protein.
This CLEC4D Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CLEC4D. It contains Human CLEC4D capture antibody, Human CLEC4D detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CLEC4D protein.
References
Johnson KD, et al. (2007) Friend of GATA-1-independent Transcriptional Repression: A Novel Mode of GATA-1 Function. Blood. 109 (12): 5230-3.Arce I, et al. (2004) The human C-type lectin CLECSF8 is a novel monocyte/macrophage endocytic receptor. Eur J Immunol. 34 (1): 210-20.Marshall AS, et al. (2006) Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation. Eur J Immunol. 36 (8): 2159-69.
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Product Notes
The CLEC4D (Catalog #AAA8118374) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CLEC4D, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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