Tricyclics ELISA Kit
Tricyclics ELISA Kit
Synonyms
Tricyclics; Tricyclics ELISA Kit; Tricyclics elisa kit
Specificity
The specificity is defined as the probability of the assay of scoring negative in the absence of the specific analyte. It is 94.8 %.
Samples
Human Serum Or Plasma
Related Product Information for Tricyclics elisa kit
Intended Uses: The Trichinella spiralis IgG-ELISA is intended for the determination of IgG class antibodies against Trichinella spiralis in human serum or plasma (citrate). For research use only.
Principle of the Assay: The immunoenzymatic determination of antibodies against Trichinella spiralis is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Trichinella spiralis ES-antigens (Excretory/Secretory antigens) to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled Protein A Conjugate is added. This conjugate binds to the captured Trichinella spiralis-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Trichinella spiralis specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance alternative 450 nm is read using an ELISA microwell plate reader!!Background/Introduction: Trichinosis (also called trichinellosis) is caused by nematodes (roundworms) of the genus Trichinella. In addition to the classical agent Trichinella spiralis, which is found worldwide in many carnivorous and omnivorous animals, four other species (T. pseudospiralis, T. nativa, T. nelsoni, and T. britovi) are recognized. Trichinosis is acquired by ingesting meat containing cysts of Trichinella. After exposure to gastric acid and pepsin, the larvae are released from the cysts and invade the small bowel mucosa where they develop into adult worms (female 2.2 mm in length, males 1.2 mm). After 1 week, the females release larvae that migrate to the striated muscles where they encyst. Encystment is completed in 4 to 5 weeks and the encysted larvae may remain viable for several years. Ingestion of the encysted larvae perpetuates the cycle. Trichinosis infection occurs worldwide, but is most common in parts of Europe and the United States. Light infections may be asymptomatic. For mild to moderate infections, most symptoms subside within a few months whereas fatigue, weakness, and diarrhoea may last for months. In severe cases, death can occur.
Principle of the Assay: The immunoenzymatic determination of antibodies against Trichinella spiralis is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Trichinella spiralis ES-antigens (Excretory/Secretory antigens) to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled Protein A Conjugate is added. This conjugate binds to the captured Trichinella spiralis-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Trichinella spiralis specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance alternative 450 nm is read using an ELISA microwell plate reader!!Background/Introduction: Trichinosis (also called trichinellosis) is caused by nematodes (roundworms) of the genus Trichinella. In addition to the classical agent Trichinella spiralis, which is found worldwide in many carnivorous and omnivorous animals, four other species (T. pseudospiralis, T. nativa, T. nelsoni, and T. britovi) are recognized. Trichinosis is acquired by ingesting meat containing cysts of Trichinella. After exposure to gastric acid and pepsin, the larvae are released from the cysts and invade the small bowel mucosa where they develop into adult worms (female 2.2 mm in length, males 1.2 mm). After 1 week, the females release larvae that migrate to the striated muscles where they encyst. Encystment is completed in 4 to 5 weeks and the encysted larvae may remain viable for several years. Ingestion of the encysted larvae perpetuates the cycle. Trichinosis infection occurs worldwide, but is most common in parts of Europe and the United States. Light infections may be asymptomatic. For mild to moderate infections, most symptoms subside within a few months whereas fatigue, weakness, and diarrhoea may last for months. In severe cases, death can occur.
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Product Notes
The Tricyclics (Catalog #AAA580072) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tricyclics, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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