Typical Testing Data/Standard Curve (for reference only)
NGF elisa kit
NGF (Mouse) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. An analyte-specific polyclonal antibody is pre-coated onto 96-well plates. The biotin conjugated second antibody is used as detection antibody. During the first incubation the standards and samples react with the analyte-specific pre-coated antibody. In a second incubation the biotin conjugated detection antibody completes the sandwich. After washing with wash buffer the TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the O.D. absorbance at 450nm in a microtiterplate reader, and calculate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample!!Background/Introduction: Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a member of the matrix metalloproteinase (MMP) family. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis, intracerebral hemorrhage, and metastasis. MMP-9 is produced by normal alveolar macrophages and granulocytes. Sundstrom et al. (2004) suggested that plasma MMP9 level may be a marker for cardiac extracellular matrix degradation, a process involved in left ventricular remodeling.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]
Uniprot Description
NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p13.1
Cellular Component: cytoplasmic membrane-bound vesicle; endosome; extracellular region; Golgi lumen
Molecular Function: growth factor activity; metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; protein binding; receptor signaling protein activity
Biological Process: activation of MAPKK activity; cell-cell signaling; induction of apoptosis via death domain receptors; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of neuron apoptosis; nerve growth factor processing; nerve growth factor receptor signaling pathway; neurite morphogenesis; neuron apoptosis; peripheral nervous system development; phosphoinositide-mediated signaling; phospholipase C activation; positive regulation of apoptosis; positive regulation of axon extension; positive regulation of axonogenesis; positive regulation of collateral sprouting; positive regulation of neuron maturation; positive regulation of protein ubiquitination; Ras protein signal transduction; regulation of axonogenesis; regulation of caspase activity; regulation of neuron differentiation; regulation of neurotransmitter secretion; regulation of release of sequestered calcium ion into cytosol; sensory perception of pain; small GTPase mediated signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway
Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V
Research Articles on NGF
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Product Notes
The NGF ngf (Catalog #AAA495409) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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