IL-1alpha elisa kit

Rat IL-1alpha ELISA KIT

Cat. Num. AAA8506118

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
• Synonyms: IL-1alpha; Rat IL-1alpha ELISA KIT; IL-1alpha elisa kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of IL1b. No significant cross-reactivity or interference between IL1b and analogues was observed.
• Sequence Length: 271

• Samples: Porcine Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Sensitivity: 0.64pg/mL
• Intra-assay Precision: 3 samples with low, middle and high level IL1b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: 3 samples with low, middle and high level IL1b were tested on 3 different plates, 8 replicates in each plate. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-1alpha elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL1b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1b present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL1b is added to the wells and binds to the combination of capture antibody-IL1b in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of IL1b present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven IL1b standard dilutions and IL1b sample concentration determined.

NCBI and Uniprot Product Information

• NCBI GI #: 27894330
• NCBI GeneID: 3552
• NCBI Accession #: NP_000566.3
• NCBI GenBank Nucleotide #: NM_000575.4
• UniProt Accession #: P01583; Q53QF9; Q7RU02
• Molecular Weight: 30,607 Da
• NCBI Official Full Name: interleukin-1 alpha
• NCBI Official Synonym Full Names: interleukin 1 alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1; IL-1 alpha

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.