HDV-IgM elisa kit
HDV-IgM ELISA Kit
Synonyms
HDV-IgM; HDV-IgM ELISA Kit; HDV-IgM elisa kit
Samples
Serum, plasma
Preparation and Storage
+4C
Related Product Information for HDV-IgM elisa kit
Principle of the Assay: This HDV IgM ELISA kit is a solid phase, two-step incubation, antibody capture assay in which polystyrene microwell strips are pre-coated with antibodies directed to human IgM (anti-u chain). The patient's serum/plasma sample is diluted and during the first incubation step, any IgM antibodies will be captured in the wells. After washing out all the other components of the sample and in particular IgG antibodies, the specific HDV IgM captured on the solid phase is detected by the addition of purified HDV antigens conjugated to horseradish peroxidase (HRP). During the second incubation step, the conjugated antigens will specifically react only with the specific HDV IgM antibodies and after washing to remove unbound conjugates, Chromogen solutions are added into the wells. In presence of the (anti-u)-(HDV-IgM)-(HDV antigen-HRP) immunocomplex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color can be measured and is proportional to the amount of antibody in the sample. Wells containing samples negative for HDVIgM remain colorless.!!Background/Introduction: This HDV-IgM kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative determination of IgM-class antibodies to hepatitis D virus in human serum or plasma. For Research Use Only
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Product Notes
The HDV-IgM (Catalog #AAA412745) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HDV-IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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