Crohn's-Check ELISA Kit
Crohn's-Check ELISA
Synonyms
Crohn's-Check; Crohn's-Check ELISA; Anti-Saccharomyces cerevisiae IgA and IgG antibodies (ASCA); Crohn's-Check elisa kit
Specificity
The microplate is coated with highly purified mannan from S. cerevisiae. No cross reactivities to other autoantigens have been found. The specificity of the test is 90% for Crohn"s disease. The sensitivity is about 68%.
Application Notes
30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time.
Samples
Human serum
Sensitivity
1.0 U/ml
Preparation and Storage
Store at 2-8 degree C
Related Product Information for Crohn's-Check elisa kit
Intended Uses: This Crohn"s-Check ELISA is a solid phase enzyme immunoassay employing highly purified mannan for the combined quantitative and qualitative detection of anti-Saccharomyces cerevisiae IgA and IgG antibodies (ASCA) in human serum.
Principle of the Assay: Samples diluted 1:101 are incubated in the microplates coated with the specific antigen. The antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMBsubstrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.
Background: Crohn's disease is one of the two major Inflammatory Bowel Diseases (IBD). IBD is an umbrella term, that covers both primary disorders causing inflammation or ulceration in the small and large intestine, Crohn's disease and ulcerative colitis. Crohn's disease affects both, the small bowel and the colon, unlike ulcerative colitis which is restricted to the colon only. The etiology is not revealed yet, although a genetic and infectious background for the disease is under discussion. Colonoscopy and ileoscopy are the established tools for detection this disease, no serology was available so far. Though Crohn's disease and ulcerative colitis share several symptoms the course of the diseases, its complications and its management are different, especially when it comes down to surgery.
Principle of the Assay: Samples diluted 1:101 are incubated in the microplates coated with the specific antigen. The antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMBsubstrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.
Background: Crohn's disease is one of the two major Inflammatory Bowel Diseases (IBD). IBD is an umbrella term, that covers both primary disorders causing inflammation or ulceration in the small and large intestine, Crohn's disease and ulcerative colitis. Crohn's disease affects both, the small bowel and the colon, unlike ulcerative colitis which is restricted to the colon only. The etiology is not revealed yet, although a genetic and infectious background for the disease is under discussion. Colonoscopy and ileoscopy are the established tools for detection this disease, no serology was available so far. Though Crohn's disease and ulcerative colitis share several symptoms the course of the diseases, its complications and its management are different, especially when it comes down to surgery.
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Product Notes
The Crohn's-Check (Catalog #AAA490232) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time. Researchers should empirically determine the suitability of the Crohn's-Check for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Crohn's-Check, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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