Complement C5a ELISA Kit
Complement C5a ELISA Kit
Applications
ELISA
Synonyms
Complement C5a; Complement C5a ELISA Kit; C5a ELISA kit; Complement C5a elisa kit
Applicable Applications for Complement C5a elisa kit
ELISA (EIA)
Application Notes
The C5a ELISA KIT is an enzyme immunoassay for the in vitro determination of human anaphylatoxin C5a and is based on the sandwich principle. Due to cross-reactivity of the monoclonal antibodies with complement factor C5, C5 in the sample is removed by precipitation prior to analysis. The resulting clear supernatant contains the C5a to be determined. During the first incubation the C5a in the sample binds to Mouse anti C5a monoclonal antibodies which are attached to the surface of the microtitration plate. Unbound constituents are then removed by washing and, in a second reaction, peroxidase conjugated monoclonal antibodies (Mab 557) are added and bound to a different epitope on C5a. The excess enzyme conjugated antibodies are removed by washing; the bound enzyme activity is then determined. The enzymatic reaction between hydrogen peroxide and chromogen is terminated by the addition of dilute sulphuric acid. The intensity of the colour intensity, which is proportional to the concentration of C5a, is determined photometrically. The concentration range of appox. 0.1 to 10 ug/L (the exact values are indicated on the labels) is covered by the standards contained in the kit. For higher concentrations the sample must be diluted with dilution reagent.
Biological Significance
Upon activation, a cascade of proteolytic enzymes releases the anaphylatoxins C3a, C4a and C5a from their respective precursors. These fragments exert various biological functions such as histamine release, smooth muscle contraction, increase in capillary permeability or immunomodulation. In addition, C5a and its degraded form C5a-desArg are highly
Biohazard Information
All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV I/II, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.
Preparation and Storage
Store at 4 degree C.
Related Product Information for Complement C5a elisa kit
ELISA kit for detection of Complement C5a in the research laboratory
Product Categories/Family for Complement C5a elisa kit
Similar Products
Product Notes
The Complement C5a (Catalog #AAA539688) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Complement C5a can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). The C5a ELISA KIT is an enzyme immunoassay for the in vitro determination of human anaphylatoxin C5a and is based on the sandwich principle. Due to cross-reactivity of the monoclonal antibodies with complement factor C5, C5 in the sample is removed by precipitation prior to analysis. The resulting clear supernatant contains the C5a to be determined. During the first incubation the C5a in the sample binds to Mouse anti C5a monoclonal antibodies which are attached to the surface of the microtitration plate. Unbound constituents are then removed by washing and, in a second reaction, peroxidase conjugated monoclonal antibodies (Mab 557) are added and bound to a different epitope on C5a. The excess enzyme conjugated antibodies are removed by washing; the bound enzyme activity is then determined. The enzymatic reaction between hydrogen peroxide and chromogen is terminated by the addition of dilute sulphuric acid. The intensity of the colour intensity, which is proportional to the concentration of C5a, is determined photometrically. The concentration range of appox. 0.1 to 10 ug/L (the exact values are indicated on the labels) is covered by the standards contained in the kit. For higher concentrations the sample must be diluted with dilution reagent. Researchers should empirically determine the suitability of the Complement C5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement C5a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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