Cholin IgG/IgM ELISA Kit
Cholin IgG/IgM ELISA
Synonyms
Cholin IgG/IgM; Cholin IgG/IgM ELISA; Phosphatidyl-cholin; Cholin IgG/IgM elisa kit
Specificity
This assay has high sensitivity and excellent specificity for detection of cholesterol. No significant cross-reactivity or interference between cholesterol and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between cholesterol and all the analogues, therefore, cross reaction may still exist in some cases.
Application Notes
30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time.
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 mmol/L.
Preparation and Storage
Store at 2-8 degree C
Related Product Information for Cholin IgG/IgM elisa kit
Intended Uses: This cholesterol ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine cholesterol. This ELISA kit for research use only!
Principle of the Assay: cholesterol ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-cholesterol antibody and an cholesterol-HRP conjugate. The assay sample and buffer are incubated together with cholesterol-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cholesterol concentration since cholesterol from samples and cholesterol-HRP conjugate compete for the anti-cholesterol antibody binding site. Since the number of sites is limited, as more sites are occupied by cholesterol from the sample, fewer sites are left to bind cholesterol-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cholesterol concentration in each sample is interpolated from this standard curve.
Principle of the Assay: cholesterol ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-cholesterol antibody and an cholesterol-HRP conjugate. The assay sample and buffer are incubated together with cholesterol-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cholesterol concentration since cholesterol from samples and cholesterol-HRP conjugate compete for the anti-cholesterol antibody binding site. Since the number of sites is limited, as more sites are occupied by cholesterol from the sample, fewer sites are left to bind cholesterol-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cholesterol concentration in each sample is interpolated from this standard curve.
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Product Notes
The Cholin IgG/IgM (Catalog #AAA490220) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time. Researchers should empirically determine the suitability of the Cholin IgG/IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cholin IgG/IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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