CD40 / TNFRSF5 elisa kit
CD40 / TNFRSF5 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. An analyte-specific polyclonal antibody is pre-coated onto 96-well plates. The biotin conjugated second antibody is used as detection antibody. During the first incubation the standards and samples react with the analyte-specific pre-coated antibody. In a second incubation the biotin conjugated detection antibody completes the sandwich. After washing with wash buffer the TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the O.D. absorbance at 450nm in a microtiterplate reader, and calculate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample!!Background/Introduction: CD30 ligand (CD30L), also known as CD153, is a cytokine that induces proliferation of T cells. The CD30L gene contains 4 exons and spans more than 17.1 kb. It has the characteristics of a type II membrane protein, with no apparent signal peptide and a transmembrane domain followed by a C-terminal extracellular domain. CD30L is expressed on the surface of B cells and found that this expression is upregulated upon CD154 (CD40L), IL4, and B-cell receptor engagement. Smith et al. (1993) found that recombinant mouse CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.
NCBI and Uniprot Product Information
Uniprot Description
CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.
Protein type: Receptor, cytokine; Membrane protein, integral
Cellular Component: cell surface; cytoplasm; external side of plasma membrane; extracellular region; extracellular space; integral to membrane; intracellular membrane-bound organelle; membrane; plasma membrane
Molecular Function: antigen binding; enzyme binding; protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding
Biological Process: activation of NF-kappaB transcription factor; B cell activation; cellular calcium ion homeostasis; defense response to protozoan; defense response to virus; immune response; immune response-regulating cell surface receptor signaling pathway; immune system process; inflammatory response; multicellular organismal development; positive regulation of B cell proliferation; positive regulation of GTPase activity; positive regulation of interleukin-12 production; positive regulation of isotype switching to IgG isotypes; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat1 protein; protein kinase B signaling cascade; regulation of apoptosis; regulation of immune response; regulation of immunoglobulin secretion; response to lipopolysaccharide
