Beta2-Microglobulin ELISA Kit | B2M elisa kit

Beta2-Microglobulin

Cat. Num. AAA590050

• Synonyms: Beta2-Microglobulin; B2M elisa kit

• Specificity: Cross-reaction with the following human serum components was not found.
• Sequence Length: 119

• Samples: Human serum, other biological samples, and Cell Culture Supernatant
• Assay Type: Sandwich
• Sensitivity: The minimal detectable dose of this B2MG assay was calculated by adding two standard deviations to the mean optical density value of 16 zero standard replicates and determining the corresponding concentration from the standard curve. The minimal detectable dose for this human B2MG assay generated by this method is 0.2?g/mL.
• Intended Uses: This Human Beta2-Microglobulin ELISA Kit is to be used for in vitro quantitative determination of Beta2-Microglobulin (B2MG) concentrations in human serum, other biological samples, and cell culture supernatant. The kit is intended for RESEARCH USE ONLY and is not for use in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for B2M elisa kit

Principle of the assay: This B2MG enzyme linked immunosorbent assay (ELISA) kit uses a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for B2MG. Standards or samples are added to the microtiter plate wells and B2MG, if present, will bind to the antibody pre-coated on the wells. A preparation of horseradish peroxidase (HRP)-conjugated B2MG antibody is added to each well. The conjugated antibody will bind to the B2MG immobilized on the plate after incubation. All unbound components will be removed by subsequent washing procedure. Next, 3, 3', 5, 5' tetramethyl-benzidine(TMB), a substrate for HRP is added to each well. The colorimetric reaction is proportional to the B2MG content in each well. The reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at the wavelength of 450nm +/- 2nm. In order to quantify the amount of B2MG present in the sample, this Human B2MG ELISA Kit includes a set of calibration standards (6 standards). The calibration standards should be assayed at the same time as the samples to allow the operator to produce a standard curve of Optical Density (O.D.) versus B2MG concentration (?g/mL). The concentration of B2MG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: Beta 2 microglobulin (B2MG) is initially discovered as a small serum protein of 12,000 Dalton. The small size allows B2MG to pass through the glomerular membrane, but it is normally completely reabsorbed in the proximal tubules. B2MG normally presents in minute amount, but increases its expressions, or adapts to the fibrillary configuration in certain pathological states. B2MG is found to be a subunit of class I major histocompatibility complex (MHC I) that is expressed on all nucleated cell excluding red blood cells. B2MG non-covalently links with the a3 domain of human leukocyte antigen (HLA) to form MHC I. Inside cytoplasm, MHC I binds to intracellular degraded foreign peptides, or self-peptides in some conditions, through the a1 and a2 variable domains of HLA. Subsequently, MHC I is anchored to the cell surface through the a3 domain of HLA. By way of this procedure, MHC I presents the engulfed foreign peptides to (CD8+) cytotoxic T-cell, thus, plays an important role in cell-mediated immunity. When binding with HFE protein, B2MG was found to increase iron uptake. B2MG is also associated with MHC I - like molecules such as CD1 and Qa. This immunoassay is a tool for investigation of the function and regulation of the protein.

Product Categories/Family for B2M elisa kit

ELISA Kits for Human Inflammatory and Autoimmune Conditions

NCBI and Uniprot Product Information

• NCBI GI #: 179318
• NCBI GeneID: 567
• Molecular Weight: 13,715 Da
• NCBI Official Full Name: beta-2-microglobulin
• NCBI Official Synonym Full Names: beta-2-microglobulin
• NCBI Official Symbol: B2M
• NCBI Protein Information: beta-2-microglobulin; beta chain of MHC class I molecules; beta-2-microglobin
• UniProt Protein Name: Beta-2-microglobulin
• Protein Family: Beta-2-microglobulin
• UniProt Gene Name: B2M
• UniProt Entry Name: B2MG_HUMAN

NCBI Description

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Uniprot Description

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; early endosome membrane; endoplasmic reticulum lumen; cytoplasm; extracellular region; plasma membrane; MHC class I protein complex; external side of plasma membrane

Molecular Function: identical protein binding; protein binding

Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Disease: Hypoproteinemia, Hypercatabolic