Typical Testing Data/Standard Curve (for reference only)
BDNF elisa kit
BDNF ELISA Kit
Synonyms
BDNF; BDNF ELISA Kit; BDNF elisa kit
Sequence Length
248
Assay Type
Competitive
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BDNF elisa kit
Intended Uses: The Barbiturate Direct ELISA Kit provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/ mass spectrometry (GS-MS) is the preferred confirmatory method. Professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
Principle of the Assay: The Barbiturate Direct ELISA Kit is based upon the competitive binding to antibody of enzyme labeled antigen and unlabeled antigen, in proportion to their concentration in the reaction mixture. A 10 ?l. aliquot of a diluted unknown specimen is incubated with a 100 ?l. dilution of enzyme (Horseradish peroxidase) labeled barbiturate derivative in micro-plate wells, coated with fixed amounts of oriented high affinity purified polyclonal antibody. The wells are washed thoroughly and a chromogenic substrate added. The color produced is stopped using a dilute acid stop solution and the wells read at 450 nm. The intensity of the color developed is inversely proportional to the concentration of drug in the sample. The technique is sensitive to 1 ng/ml. The Barbiturate Direct ELISA Kit avoids extraction of urine or blood sample for measurement. It employs a polyclonal high affinity, purified Barbiturate antibody. Due to the proprietary method of orienting the antibody on the polystyrene micro-plate much higher sensitivity is achieved compared to passive adsorption. This results in extremely small sample size reducing matrix effects and interference with binding proteins(s) or other macromolecules.
Principle of the Assay: The Barbiturate Direct ELISA Kit is based upon the competitive binding to antibody of enzyme labeled antigen and unlabeled antigen, in proportion to their concentration in the reaction mixture. A 10 ?l. aliquot of a diluted unknown specimen is incubated with a 100 ?l. dilution of enzyme (Horseradish peroxidase) labeled barbiturate derivative in micro-plate wells, coated with fixed amounts of oriented high affinity purified polyclonal antibody. The wells are washed thoroughly and a chromogenic substrate added. The color produced is stopped using a dilute acid stop solution and the wells read at 450 nm. The intensity of the color developed is inversely proportional to the concentration of drug in the sample. The technique is sensitive to 1 ng/ml. The Barbiturate Direct ELISA Kit avoids extraction of urine or blood sample for measurement. It employs a polyclonal high affinity, purified Barbiturate antibody. Due to the proprietary method of orienting the antibody on the polystyrene micro-plate much higher sensitivity is achieved compared to passive adsorption. This results in extremely small sample size reducing matrix effects and interference with binding proteins(s) or other macromolecules.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
27,051 Da
NCBI Official Full Name
BDNF, partial
UniProt Protein Name
BDNF
Protein Family
UniProt Gene Name
BDNF
UniProt Entry Name
D0VPU4_ONCMY
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Product Notes
The BDNF bdnf (Catalog #AAA495331) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BDNF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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