Typical Standard Curve/Testing Data
AAV elisa kit
AAV ELISA Kit
Applications
ELISA
Synonyms
AAV; AAV ELISA Kit; Adeno Associated Virus ELISA Kit; AAV Titration ELISA Kit; AAV elisa kit
Applicable Applications for AAV elisa kit
ELISA (EIA)
Assay Type
Quantitative Sandwich
Preparation and Storage
Store at 4 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for AAV elisa kit
Principle of the Assay: The assay is based on the sandwich ELISA technique. A monoclonal antibody specific for a conformational epitope on assembled AAV 2 capsids is coated onto microtiter strips and is used to capture AAV 2 particles from the specimen. Captured AAV particles are detected in two steps. First a biotin-conjugated monoclonal antibody to AAV 2 is bound to the immune complex. In the second step streptavidin peroxidase conjugate reacts with the biotin molecules. Addition of substrate solution results in a color reaction which is proportional to the amount of specifically bound viral particles. The absorbance is measured photometrically at 450 nm. The kit control provided contains an AAV 2 particle preparation of empty capsids. It shows a typical titration curve when used in dilutions of steps of two (Fig. 1). It allows the quantitative determination of samples of an unknown particle titre (immunological titer) and the calibration of an inhouse AAV 2 preparation (e.g. infectious titer, transducing units, DNA dot blot titer) (Table 1).
Background/Introduction: Adeno-associated virus (AAV) is a non pathogenic ssDNA virus that is a topic of intense study in gene therapy. The virus transduces a wide variety of dividing and non-dividing cells showing long-term gene expression with no cellular immune response. AAV has been used in several clinical trials (e.g. FIX, CFTR, Parkinsons's, Cana????an disease) showing no serious vector-related adverse effects. Methods for the characterisation of AAV preparations currently include titration ELISA, realtime PCR, DNA dot blot, determination of transducing units, infectious center assay, SDSPAGE or electron microscopy. Immunotitration by AAV 2 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV 2 wt virions, AAV 2 recombinant virions or assembled and intact empty AAV 2 capsids.
Background/Introduction: Adeno-associated virus (AAV) is a non pathogenic ssDNA virus that is a topic of intense study in gene therapy. The virus transduces a wide variety of dividing and non-dividing cells showing long-term gene expression with no cellular immune response. AAV has been used in several clinical trials (e.g. FIX, CFTR, Parkinsons's, Cana????an disease) showing no serious vector-related adverse effects. Methods for the characterisation of AAV preparations currently include titration ELISA, realtime PCR, DNA dot blot, determination of transducing units, infectious center assay, SDSPAGE or electron microscopy. Immunotitration by AAV 2 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV 2 wt virions, AAV 2 recombinant virions or assembled and intact empty AAV 2 capsids.
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Product Notes
The AAV (Catalog #AAA539692) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AAV can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the AAV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AAV, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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