Canine Endothelial-leukocyte adhesion molecule 1 ELISA Kit | ELAM-1 elisa kit

Canine Endothelial-leukocyte adhesion molecule 1 ELISA Kit

Cat. Num. AAA752577

• Gene Names: SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
• Reactivity: Canine
• Synonyms: Endothelial-leukocyte adhesion molecule 1; Canine Endothelial-leukocyte adhesion molecule 1 ELISA Kit; Canine ELAM-1 ELISA Kit; ELAM-1 ELISA Kit; ELAM-1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ELAM-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ELAM-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELAM-1 elisa kit

Principle of the assay: ELAM-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ELAM-1 antibody and an ELAM-1-HRP conjugate. The assay sample and buffer are incubated together with ELAM-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ELAM-1 concentration since ELAM-1 from samples and ELAM-1-HRP conjugate compete for the anti-ELAM-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ELAM-1 from the sample, fewer sites are left to bind ELAM-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ELAM-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ELAM-1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 187960042
• NCBI GeneID: 6401
• NCBI Accession #: NP_000441.2
• NCBI GenBank Nucleotide #: NM_000450.2
• UniProt Accession #: P16581; P16111; A2RRD6
• Molecular Weight: 66,655 Da
• NCBI Official Full Name: E-selectin
• NCBI Official Synonym Full Names: selectin E
• NCBI Official Symbol: SELE
• NCBI Official Synonym Symbols: ELAM; ESEL; CD62E; ELAM1; LECAM2
• NCBI Protein Information: E-selectin; ELAM-1; endothelial adhesion molecule 1; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2
• UniProt Protein Name: E-selectin
• UniProt Gene Name: SELE
• UniProt Synonym Gene Names: ELAM1; ELAM-1; LECAM2
• UniProt Entry Name: LYAM2_HUMAN

NCBI Description

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; integral to membrane; plasma membrane; coated pit; caveola; lipid raft

Molecular Function: protein binding; transmembrane receptor activity; phospholipase binding; sialic acid binding

Biological Process: positive regulation of leukocyte migration; actin filament-based process; positive regulation of receptor internalization; calcium-mediated signaling; response to lipopolysaccharide; leukocyte migration during inflammatory response; heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; regulation of inflammatory response; inflammatory response; leukocyte tethering or rolling; blood coagulation; leukocyte migration

Disease: Hypertension, Essential