Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Recombinant Protein | EIF4EBP1 recombinant protein
Recombinant Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)
Gene Names
Eif4ebp1; 4e-bp1; PHAS-I; AA959816
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1); Recombinant Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1); EIF4EBP1 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with a N-terminal His-tag, its sequence is listed below.
MGHHHHHHSGSEF-SAGSSCSQT PSRAIPTRRV ALGDGVQLPP GDYSTTPGGT LFSTTPGGTR IIYDRKFLME CRNSPVAKTP PKDLPAIPGV TSPTSDEPPM QASQSQLPSS PEDKRAGGEE SQFEMDI
MGHHHHHHSGSEF-SAGSSCSQT PSRAIPTRRV ALGDGVQLPP GDYSTTPGGT LFSTTPGGTR IIYDRKFLME CRNSPVAKTP PKDLPAIPGV TSPTSDEPPM QASQSQLPSS PEDKRAGGEE SQFEMDI
Sequence Length
117
Applicable Applications for EIF4EBP1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Ser2~Ile117 (Accession # Q60876) with a N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13.7kDa
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
Eif4ebp1
NCBI Official Synonym Symbols
4e-bp1; PHAS-I; AA959816
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
Protein Family
UniProt Gene Name
Eif4ebp1
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I
UniProt Entry Name
4EBP1_MOUSE
Uniprot Description
4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.
Protein type: Translation; Translation initiation
Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus
Molecular Function: protein binding; translation repressor activity; translation initiation factor binding; eukaryotic initiation factor 4E binding
Biological Process: regulation of translation; negative regulation of translational initiation; TOR signaling pathway; negative regulation of translation; insulin receptor signaling pathway; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; regulation of translational initiation
Research Articles on EIF4EBP1
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Product Notes
The EIF4EBP1 eif4ebp1 (Catalog #AAA2009316) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the EIF4EBP1 eif4ebp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with a N-terminal His-tag, its sequence is listed below. MGHHHHHHSG SEF-SAGSSC SQT PSRAIPTRRV ALGDGVQLPP GDYSTTPGGT LFSTTPGGTR IIYDRKFLME CRNSPVAKTP PKDLPAIPGV TSPTSDEPPM QASQSQLPSS PEDKRAGGEE SQFEMDI. It is sometimes possible for the material contained within the vial of "Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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