EIF4A2 recombinant protein

Recombinant EIF4A2 protein

Cat. Num. AAA389266

• Gene Names: EIF4A2; DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
• Synonyms: EIF4A2; Recombinant EIF4A2 protein; Eukaryotic Translation Initiation Factor 4A2; DDX2B; EIF4A2 recombinant protein

• Host: E Coli
• Form/Format: Recombinant EIF4A2 protein is supplied in 25mM Tris-HCl pH8.0, 500mM NaCl, 10% glycerol, and 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Protein Details: Recombinant EIF4A2 protein was expressed in E Coli as the full length protein (accession number NP_001958.2) with an N-terminal 6xHis-tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant EIF4A2 protein 10% SDS-PAGE gel MW: 49.9 kDa Purity: >90%)

Related Product Information for EIF4A2 recombinant protein

Short Description: EIF4A2 (Eukaryotic Translation Initiation Factor 4A, Isoform 2), also called EIF4F or DDX2B, is an ATP dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to the ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome, ubiquitous with high expression in skeletal muscle and ovary. Diseases associated with EIF4A2 include Retinitis Pigmentosa 29 and X-Linked Hereditary Ataxia. Among its related pathways are the TGFb pathway and Apoptotic pathways in Synovial Fibroblasts.

Background: EIF4A2 (Eukaryotic Translation Initiation Factor 4A, Isoform 2), also called EIF4F or DDX2B, is an ATP dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to the ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome,ubiquitous with high expression in skeletal muscle and ovary. Diseases associated with EIF4A2 include Retinitis Pigmentosa 29 and X-Linked Hereditary Ataxia. Among its related pathways are the TGFb pathway and Apoptotic pathways in Synovial Fibroblasts.

Product Categories/Family for EIF4A2 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 83700235
• NCBI GeneID: 1974
• NCBI Accession #: NP_001958.2
• NCBI GenBank Nucleotide #: NM_001967.3
• UniProt Accession #: Q14240; Q53XJ6; Q96B90; Q96EA8; D3DNU9
• Molecular Weight: 46,489 Da
• NCBI Official Full Name: eukaryotic initiation factor 4A-II
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4A2
• NCBI Official Symbol: EIF4A2
• NCBI Official Synonym Symbols: DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
• NCBI Protein Information: eukaryotic initiation factor 4A-II
• UniProt Protein Name: Eukaryotic initiation factor 4A-II
• Protein Family: Eukaryotic initiation factor
• UniProt Gene Name: EIF4A2
• UniProt Synonym Gene Names: DDX2B; EIF4F; eIF-4A-II; eIF4A-II
• UniProt Entry Name: IF4A2_HUMAN

Uniprot Description

EIF4A2: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Belongs to the DEAD box helicase family. eIF4A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation; Translation initiation; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: perinuclear region of cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; translation initiation factor activity; ATPase activity; helicase activity; ATP binding

Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; viral reproduction; cytokine and chemokine mediated signaling pathway; translational initiation; gene expression; regulation of translational initiation; mRNA catabolic process, deadenylation-dependent decay