Typical Testing Data/Standard Curve (for reference only)
Human Protein argonaute-2 ELISA Kit | EIF2C2 elisa kit
Human Protein argonaute-2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Uniprot Description
AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).
Protein type: EC 3.1.26.n2; RNA processing; RNA-binding
Chromosomal Location of Human Ortholog: 8q24
Cellular Component: cytoplasm; cytosol; dendrite; membrane; mRNA cap complex; nucleoplasm; nucleus; polysome; ribonucleoprotein complex
Molecular Function: double-stranded RNA binding; endoribonuclease activity; metal ion binding; miRNA binding; mRNA binding; protein binding; protein C-terminus binding; RNA 7-methylguanosine cap binding; single-stranded RNA binding; siRNA binding; translation initiation factor activity
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; gene expression; innate immune response; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; negative regulation of translational initiation; nerve growth factor receptor signaling pathway; Notch signaling pathway; phosphoinositide-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; pre-microRNA processing; RNA interference, siRNA loading onto RISC; RNA secondary structure unwinding; RNA-mediated gene silencing; RNA-mediated posttranscriptional gene silencing; transcription, DNA-dependent; translation; translational initiation
Research Articles on EIF2C2
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Product Notes
The Human EIF2C2 ago2 (Catalog #AAA2882405) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882405 ELISA Kit recognizes Human EIF2C2. It is sometimes possible for the material contained within the vial of "Protein argonaute-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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