Human eukaryotic translation initiation factor 2C, 2 ELISA Kit | EIF2C2 elisa kit

Human Protein argonaute-2, EIF2C2 ELISA Kit

Cat. Num. AAA910054

• Gene Names: AGO2; Q10; EIF2C2
• Reactivity: Human
• Synonyms: eukaryotic translation initiation factor 2C; 2; Human Protein argonaute-2; EIF2C2 ELISA Kit; Human Protein argonaute-2 (EIF2C2) ELISA kit; AGO2; MGC3183; Q10; argonaute 2; EIF2C2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human EGFL6. No significant cross-reactivity or interference between human EGFL6 and analogues was observed.
• Sequence Length: 825

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 78 pg/ml-5000 pg/ml
• Sensitivity: 19.5 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EIF2C2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGFL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGFL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 257467482
• NCBI GeneID: 27161
• NCBI Accession #: NP_001158095.1
• NCBI GenBank Nucleotide #: NM_001164623.1
• UniProt Accession #: Q9UKV8; Q8TCZ5; Q8WV58; Q96ID1
• Molecular Weight: 97,208 Da
• NCBI Official Full Name: protein argonaute-2 isoform 2
• NCBI Official Synonym Full Names: argonaute RISC catalytic component 2
• NCBI Official Symbol: AGO2
• NCBI Official Synonym Symbols: Q10; EIF2C2
• NCBI Protein Information: protein argonaute-2; PPD; hAgo2; eIF2C 2; eIF-2C 2; argonaute2; argonaute 2; protein slicer; PAZ Piwi domain protein; eukaryotic translation initiation factor 2C, 2
• UniProt Protein Name: Protein argonaute-2
• UniProt Gene Name: AGO2
• UniProt Synonym Gene Names: EIF2C2; Argonaute2; hAgo2; eIF-2C 2; eIF2C 2; PPD
• UniProt Entry Name: AGO2_HUMAN

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: RNA processing; RNA-binding; EC 3.1.26.n2

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: nucleoplasm; polysome; mRNA cap complex; membrane; cytoplasm; ribonucleoprotein complex; cytosol

Molecular Function: mRNA binding; endoribonuclease activity; protein binding; miRNA binding; siRNA binding; metal ion binding; translation initiation factor activity; RNA 7-methylguanosine cap binding

Biological Process: epidermal growth factor receptor signaling pathway; Notch signaling pathway; negative regulation of translational initiation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; translation; transcription, DNA-dependent; RNA interference, siRNA loading onto RISC; nerve growth factor receptor signaling pathway; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; post-embryonic development; regulation of transcription, DNA-dependent; pre-microRNA processing; translational initiation; innate immune response; RNA-mediated gene silencing; gene expression; posttranscriptional gene silencing