Human Eukaryotic elongation factor 2 kinase ELISA Kit | EEF2K elisa kit

Human Eukaryotic elongation factor 2 kinase ELISA Kit

Cat. Num. AAA2884402

• Gene Names: EEF2K; eEF-2K; HSU93850
• Reactivity: Human
• Synonyms: Eukaryotic elongation factor 2 kinase; Human Eukaryotic elongation factor 2 kinase ELISA Kit; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; EEF2K; 2.7.11.20; EEF2K elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Eukaryotic elongation factor 2 kinase,eEF-2 kinase
• Sequence Length: 725

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 78-5000 pg/mL
• Sensitivity: <39.28 pg/mL
• Intra-assay Precision: Intra-Assay CV: <=6.6%
• Inter-assay Precision: Inter-Assay CV: <=10.3%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EEF2K elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9558749
• NCBI GeneID: 29904
• NCBI Accession #: NP_037434.1
• NCBI GenBank Nucleotide #: NM_013302.3
• UniProt Accession #: O00418; Q8N588
• Molecular Weight: 82,144 Da
• NCBI Official Full Name: eukaryotic elongation factor 2 kinase
• NCBI Official Synonym Full Names: eukaryotic elongation factor 2 kinase
• NCBI Official Symbol: EEF2K
• NCBI Official Synonym Symbols: eEF-2K; HSU93850
• NCBI Protein Information: eukaryotic elongation factor 2 kinase
• UniProt Protein Name: Eukaryotic elongation factor 2 kinase
• Protein Family: Eukaryotic elongation factor 2 kinase
• UniProt Gene Name: EEF2K
• UniProt Synonym Gene Names: eEF-2 kinase; eEF-2K
• UniProt Entry Name: EF2K_HUMAN

NCBI Description

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle

Protein type: Protein kinase, atypical; EC 2.7.11.20; Kinase, protein; Translation; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; Alpha family; eEF2K subfamily

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytoplasm; cytosol; postsynaptic density

Molecular Function: ATP binding; calcium ion binding; calmodulin binding; elongation factor-2 kinase activity; protein kinase activity; translation factor activity, nucleic acid binding

Biological Process: insulin receptor signaling pathway; negative regulation of apoptosis; positive regulation of endocytosis; positive regulation of synaptogenesis; protein amino acid autophosphorylation; regulation of protein amino acid autophosphorylation; translational elongation