EEF1G recombinant protein

EEF1G, 1-437aa, Human, His tag, E Coli

Cat. Num. AAA205917

• Gene Names: EEF1G; EF1G; GIG35
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: EEF1G; 1-437aa; Human; His tag; E Coli; Elongation factor 1-gamma; EF1G; GIG35; EF-1-gamma; Elongation factor 1 gamma; PRO1608; EEF1G recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAGTLY TYPENWRAFK ALIAAQYSGA QVRVLSAPPH FHFGQTNRTP EFLRKFPAGK VPAFEGDDGF CVFESNAIAY YVSNEELRGS TPEAAAQVVQ WVSFADSDIV PPASTWVFPT LGIMHHNKQA TENAKEEVRR ILGLLDAYLK TRTFLVGERV TLADITVVCT LLWLYKQVLE PSFRQAFPNT NRWFLTCINQ PQFRAVLGEV KLCEKMAQFD AKKFAETQPK KDTPRKEKGS REEKQKPQAE RKEEKKAAAP APEEEMDECE QALAAEPKAK DPFAHLPKST FVLDEFKRKY SNEDTLSVAL PYFWEHFDKD GWSLWYSEYR FPEELTQTFM SCNLITGMFQ RLDKLRKNAF ASVILFGTNN SSSISGVWVF RGQELAFPLS PDWQVDYESY TWRKLDPGSE ETQTLVREYF SWEGAFQHVG KAFNQGKIFK
• Sequence Length: 437

• Applicable Applications for EEF1G recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for EEF1G recombinant protein

Elongation factor 1-gamma, also known as EEF1G, is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. Increased expression of EEF1G is associated with pancreatic cancer, suggesting a possible role for EEF1G in the oncogenic transformation process. Recombinant human EEF1G protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for EEF1G recombinant protein

Epigenomics (Transcription & Translation)

References

Sanders J., et al. (1992) Nucleic Aciads Res. 20: 5907-5910.; Koonin E V., et al. (1994) Protein Sci. 3: 2045-2054.

NCBI and Uniprot Product Information

• NCBI GI #: 4503481
• NCBI GeneID: 1937
• NCBI Accession #: NP_001395
• NCBI GenBank Nucleotide #: NM_001404.4
• UniProt Accession #: P26641; Q6PJ62; Q6PK31; Q96CU2; Q9P196; B4DTG2
• Molecular Weight: 52.5 kDa (460aa)
• NCBI Official Full Name: elongation factor 1-gamma
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 gamma
• NCBI Official Symbol: EEF1G
• NCBI Official Synonym Symbols: EF1G; GIG35
• NCBI Protein Information: elongation factor 1-gamma
• UniProt Protein Name: Elongation factor 1-gamma
• Protein Family: Elongation factor
• UniProt Gene Name: EEF1G
• UniProt Synonym Gene Names: EF1G; EF-1-gamma

NCBI Description

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq, Jul 2008]

Uniprot Description

Probably plays a role in anchoring the complex to other cellular components.