EEF1E1 recombinant protein

EEF1E1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121608

• Gene Names: EEF1E1; P18; AIMP3
• Purity: >85% as determined by SDS-PAGE
• Synonyms: EEF1E1; EEF1E1 Protein; Human; Recombinant (His Tag); Human EEF1E1/AIMP3/p18 Protein (His Tag); AIMP3 Protein; P18 Protein; eukaryotic translation elongation factor 1 epsilon 1; EEF1E1 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% Glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His174

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human EEF1E1 (NP_004271.1)(Met1-His174) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EEF1E1 recombinant protein

Background: EEF1E1, also known as AIMP3 and p18, is a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, EEF1E1 is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. It is comprised of a bifunctional glutamyl-prolyl-tRNA synthase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl and aspartyl-tRNA synthases, and three auxiliary proteins, EEF1E1/p18, AIMP2/p38 and AIMP1/p43. EEF1E1 also plays a positive role in ATM/ATR-mediated p53 activation.

References

Mao M. et al., 1998, Proc Natl Acad Sci. 95 (14): 8175-80.Quevillon S. et al., 1999, J Mol Biol. 285 (1): 183-95.Ahn HC. et al., 2003, FEBS Lett. 542 (1-3): 119-24.

NCBI and Uniprot Product Information

• NCBI GI #: 4758862
• NCBI GeneID: 9521
• NCBI Accession #: NP_004271.1
• NCBI GenBank Nucleotide #: NM_004280.4
• UniProt Accession #: O43324; Q5THS2; C9JLK5
• Molecular Weight: 15,548 Da
• NCBI Official Full Name: eukaryotic translation elongation factor 1 epsilon-1 isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 epsilon 1
• NCBI Official Symbol: EEF1E1
• NCBI Official Synonym Symbols: P18; AIMP3
• NCBI Protein Information: eukaryotic translation elongation factor 1 epsilon-1; ARS-interacting multifunctional protein 3; multisynthase complex auxiliary component p18; p18 component of aminoacyl-tRNA synthetase complex; aminoacyl tRNA synthetase complex-interacting multifunction
• UniProt Protein Name: Eukaryotic translation elongation factor 1 epsilon-1
• Protein Family: Eukaryotic translation elongation factor
• UniProt Gene Name: EEF1E1
• UniProt Synonym Gene Names: AIMP3; P18
• UniProt Entry Name: MCA3_HUMAN

NCBI Description

This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010]

Uniprot Description

EEF1E1: Positive modulator of ATM response to DNA damage.

Protein type: Translation; Translation elongation

Chromosomal Location of Human Ortholog: 6p24.3

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; positive regulation of apoptosis; gene expression; positive regulation of DNA damage response, signal transduction by p53 class mediator