Human Elongation factor 1-alpha 1 ELISA Kit | EEF1A1 elisa kit

Human Elongation factor 1-alpha 1 ELISA Kit

Cat. Num. AAA763892

• Gene Names: EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
• Reactivity: Human
• Synonyms: Elongation factor 1-alpha 1; Human Elongation factor 1-alpha 1 ELISA Kit; EEF1A1 (Elongation factor 1-alpha 1)/EEF1A/EF1A/LENG7/EF-1-alpha-1/EF-Tu/eEF1A-1/Leukocyte receptor cluster member 7/Elongation factor Tu/Eukaryotic elongation factor 1 A-1; EEF1A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EEF1A1. No significant cross-reactivity or interference between EEF1A1 and analogues was observed.
• Sequence Length: 462

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for EEF1A1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503471
• NCBI GeneID: 1915
• NCBI Accession #: NP_001393.1
• NCBI GenBank Nucleotide #: NM_001402.5
• Molecular Weight: 47,869 Da
• NCBI Official Full Name: elongation factor 1-alpha 1
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 alpha 1
• NCBI Official Symbol: EEF1A1
• NCBI Official Synonym Symbols: CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
• NCBI Protein Information: elongation factor 1-alpha 1
• UniProt Protein Name: Elongation factor 1-alpha 1
• Protein Family: Elongation factor
• UniProt Gene Name: EEF1A1
• UniProt Synonym Gene Names: EEF1A; EF1A; LENG7; EF-1-alpha-1; EF-Tu; eEF1A-1
• UniProt Entry Name: EF1A1_HUMAN

NCBI Description

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF1A1: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Protein type: Nucleolus; Translation; Translation elongation

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: cortical actin cytoskeleton; cytoplasm; cytosol; eukaryotic translation elongation factor 1 complex; extracellular space; membrane; nucleolus; nucleus

Molecular Function: GTP binding; GTPase activity; protein binding; protein kinase binding; translation elongation factor activity; tRNA binding

Biological Process: cellular protein metabolic process; gene expression; regulation of transcription, DNA-dependent; transcription, DNA-dependent; translation; translational elongation