Typical Testing Data/Standard Curve (for reference only)
Bovine Endothelin-1 ELISA Kit | EDN1 elisa kit
Bovine Endothelin-1 ELISA Kit
Reactivity
Bovine
Synonyms
Endothelin-1; Bovine Endothelin-1 ELISA Kit; ET-1; Preproendothelin-1; PPET1; EDN1; EDN1 elisa kit
Reactivity
Bovine
Specificity
This assay recognizes recombinant and natural Bovine ET-1. No significant cross-reactivity or interference was observed.
Sequence Length
202
Samples
Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Assay Type
Competitive (Quantitative)
Detection Range
15.6-1000 pg/mL.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EDN1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovine ET-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ET-1, During the reaction, ET-1 in the sample or standard competes with a fixed amount of biotin-labeled ET-1 for sites on a pre-coated Monoclonal antibody specific to ET-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ET-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ET-1, During the reaction, ET-1 in the sample or standard competes with a fixed amount of biotin-labeled ET-1 for sites on a pre-coated Monoclonal antibody specific to ET-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ET-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,977 Da
NCBI Official Full Name
endothelin-1
NCBI Official Symbol
EDN1
NCBI Protein Information
endothelin-1
UniProt Protein Name
Endothelin-1
Protein Family
UniProt Gene Name
EDN1
UniProt Synonym Gene Names
ET-1; PPET1
UniProt Entry Name
EDN1_BOVIN
Uniprot Description
Endothelins are endothelium-derived vasoconstrictor peptides.
Research Articles on EDN1
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Product Notes
The Bovine EDN1 edn1 (Catalog #AAA2883886) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2883886 ELISA Kit recognizes Bovine EDN1. It is sometimes possible for the material contained within the vial of "Endothelin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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