Enoyl CoA Hydratase 1 Recombinant Protein | ECH1 recombinant protein

Recombinant Human Enoyl CoA Hydratase 1, Peroxisomal

Cat. Num. AAA144117

• Gene Names: ECH1; HPXEL
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Enoyl CoA Hydratase 1; Recombinant Human Enoyl CoA Hydratase 1; Peroxisomal; ECH1 Human; Peroxisomal Human Recombinant; peroxisomal; enoyl Coenzyme A hydratase 1; ECH1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The ECH1 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 50mM NaCl, and 10% glycerol.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MTGSSAQEAA SGVALGEAPD HSYESLRVTS AQKHVLHVQL NRPNKRNAMN KVFWREMVEC FNKISRDADC RAVVISGAGK MFTAGIDLMD MASDILQPKG DDVARISWYL RDIITRYQET FNVIERCPKP VIAAVHGGCI GGGVDLVTAC DIRYCAQDAF FQVKEVDVGL AADVGTLQRL PKVIGNQSLV NELAFTARKM MADEALGSGL VSRVFPDKEV MLDAALALAA EISSKSPVAV QSTKVNLLYS RDHSVAESLN YVASWNMSML QTQDLVKSVQ ATTENKELKT VTFSKL
• Sequence Length: 328

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for ECH1 recombinant protein

Description: ECH1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 316 amino acids (34-328a.a.) and having a molecular mass of 34.4kDa.ECH1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: ECH1 is a member of the hydratase/isomerase superfamily. ECH1 demonstrates high sequence similarity to enoyl-coenzyme A (CoA) hydratases of more than a few species, mostly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence, localizes to both the peroxisome and the mitochondria. peroxisomal takes part in the auxiliary step of the fatty acid beta-oxidation pathway specifically functioning to catalyze the isomerization of 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-transdienoyl-CoA.

Product Categories/Family for ECH1 recombinant protein

Enzymes; Hydratase

NCBI and Uniprot Product Information

• NCBI GI #: 70995211
• NCBI GeneID: 1891
• NCBI Accession #: NP_001389.2
• NCBI GenBank Nucleotide #: NM_001398.2
• UniProt Accession #: Q13011; Q8WVX0; Q96EZ9; A8K745
• Molecular Weight: 35,816 Da
• NCBI Official Full Name: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
• NCBI Official Synonym Full Names: enoyl CoA hydratase 1, peroxisomal
• NCBI Official Symbol: ECH1
• NCBI Official Synonym Symbols: HPXEL
• NCBI Protein Information: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; enoyl Coenzyme A hydratase 1, peroxisomal; peroxisomal enoyl-CoA hydratase 1
• UniProt Protein Name: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
• UniProt Gene Name: ECH1
• UniProt Entry Name: ECH1_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]

Uniprot Description

ECH1: Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA. Belongs to the enoyl-CoA hydratase/isomerase family.

Protein type: Amino Acid Metabolism - tyrosine; Mitochondrial; Isomerase; EC 5.3.3.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: membrane; mitochondrion; peroxisome

Molecular Function: protein binding; isomerase activity; receptor binding

Biological Process: fatty acid beta-oxidation