Typical Testing Data/Standard Curve (for reference only)
Porcine Eosinophil chemotactic factor ELISA Kit | ECF elisa kit
Porcine Eosinophil chemotactic factor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ECF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ECF antibody and an ECF-HRP conjugate. The assay sample and buffer are incubated together with ECF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ECF concentration since ECF from samples and ECF-HRP conjugate compete for the anti-ECF antibody binding site. Since the number of sites is limited, as more sites are occupied by ECF from the sample, fewer sites are left to bind ECF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECF concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. [provided by RefSeq, Sep 2014]
Uniprot Description
CCL11: In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; chemokine activity
Biological Process: chronic inflammatory response; mast cell chemotaxis; response to virus; actin filament organization; cytoskeleton organization and biogenesis; chemotaxis; signal transduction; protein amino acid phosphorylation; cellular calcium ion homeostasis; regulation of cell shape; positive regulation of angiogenesis; response to radiation; positive regulation of actin filament polymerization; eosinophil chemotaxis; positive regulation of endothelial cell proliferation; immune response; cell adhesion; inflammatory response; positive regulation of cell migration
Disease: Asthma, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on ECF
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Product Notes
The Porcine ECF ccl11 (Catalog #AAA740576) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA740576 ELISA Kit recognizes Porcine ECF. It is sometimes possible for the material contained within the vial of "Eosinophil chemotactic factor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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