Human Endothelin-converting enzyme 1 ELISA Kit | ECE1 elisa kit

Human Endothelin-converting enzyme 1 ELISA Kit

Cat. Num. AAA762560

• Gene Names: ECE1; ECE
• Reactivity: Human
• Synonyms: Endothelin-converting enzyme 1; Human Endothelin-converting enzyme 1 ELISA Kit; ECE1/ECE/ECE-1/endothelin converting enzyme 1/endothelin-converting enzyme 1; ECE1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ECE1. No significant cross-reactivity or interference between ECE1 and analogues was observed.
• Sequence Length: 758

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ECE1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 164519136
• NCBI GeneID: 1889
• NCBI Accession #: NP_001106818.1
• NCBI GenBank Nucleotide #: NM_001113347.1
• Molecular Weight: 87,022 Da
• NCBI Official Full Name: endothelin-converting enzyme 1 isoform 3
• NCBI Official Synonym Full Names: endothelin converting enzyme 1
• NCBI Official Symbol: ECE1
• NCBI Official Synonym Symbols: ECE
• NCBI Protein Information: endothelin-converting enzyme 1
• UniProt Protein Name: Endothelin-converting enzyme 1
• Protein Family: Endothelin-converting enzyme
• UniProt Gene Name: ECE1
• UniProt Synonym Gene Names: ECE-1
• UniProt Entry Name: ECE1_HUMAN

NCBI Description

The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

ECE1: Converts big endothelin-1 to endothelin-1. Defects in ECE1 are a cause of Hirschsprung disease cardiac defects and autonomic dysfunction (HSCRCDAD). It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction. Belongs to the peptidase M13 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; EC 3.4.24.71; Cell surface; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: early endosome; endosome; external side of plasma membrane; integral to membrane; lysosomal membrane; membrane; perinuclear region of cytoplasm; plasma membrane; vesicle

Molecular Function: endopeptidase activity; metal ion binding; metalloendopeptidase activity; peptide hormone binding; protein binding; protein homodimerization activity

Biological Process: apoptosis; ear development; embryonic digit morphogenesis; heart development; hormone catabolic process; peptide hormone processing; pharyngeal system development; positive regulation of cAMP biosynthetic process; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of receptor recycling; protein processing; regulation of systemic arterial blood pressure by endothelin; regulation of vasoconstriction; regulation of vasodilation; response to cadmium ion; response to drug; response to hypoxia

Disease: Hirschsprung Disease, Cardiac Defects, And Autonomic Dysfunction; Hypertension, Essential