Rat E1/ubiquitin-activating enzyme ELISA Kit | E1/UBAE elisa kit

Rat E1/ubiquitin-activating enzyme ELISA Kit

Cat. Num. AAA729134

• Gene Names: UBA5; THIFP1; UBE1DC1
• Reactivity: Rat
• Synonyms: E1/ubiquitin-activating enzyme; Rat E1/ubiquitin-activating enzyme ELISA Kit; E1/UBAE elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 1.0-25 ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This E1/UBAE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat E1/UBAE. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for E1/UBAE elisa kit

Principle of the assay: E1/UBAE ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for E1/UBAE. Standards or samples are then added to the microtiter plate wells and E1/UBAE if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of E1/UBAE present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for E1/UBAE are added to each well to “sandwich” the E1/UBAE immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain E1/UBAE and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E1/UBAE concentration in each sample is interpolated from this standard curve.

Product Categories/Family for E1/UBAE elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 46389771
• NCBI GeneID: 79876
• Molecular Weight: 38,537 Da
• NCBI Official Full Name: Ubiquitin activating enzyme
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 5
• NCBI Official Symbol: UBA5
• NCBI Official Synonym Symbols: THIFP1; UBE1DC1
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 5; UFM1-activating enzyme; ubiquitin-activating enzyme 5; UBA5, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-activating enzyme E1 domain-containing protein 1
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 5
• UniProt Gene Name: UBA5
• UniProt Synonym Gene Names: UBE1DC1; Ubiquitin-activating enzyme 5
• UniProt Entry Name: UBA5_HUMAN

NCBI Description

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE1DC1: E1-like enzyme which activates UFM1 and SUMO2. Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; metal ion binding; ATP binding

Biological Process: regulation of estrogen receptor signaling pathway