Porcine E1/ubiquitin-activating enzyme ELISA Kit | E1/UBAE elisa kit

Porcine E1/ubiquitin-activating enzyme ELISA Kit

Cat. Num. AAA740715

• Gene Names: UBA5; THIFP1; UBE1DC1
• Reactivity: Porcine
• Synonyms: E1/ubiquitin-activating enzyme; Porcine E1/ubiquitin-activating enzyme ELISA Kit; E1/UBAE elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for E1/UBAE elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This E1/UBAE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine E1/UBAE. This ELISA kit for research use only! 2. PRINCIPLE OF THE ASSAY E1/UBAE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-E1/UBAE antibody and an E1/UBAE-HRP conjugate. The assay sample and buffer are incubated together with E1/UBAE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E1/UBAE concentration since E1/UBAE from samples and E1/UBAE-HRP conjugate compete for the anti-E1/UBAE antibody binding site. Since the number of sites is limited, as more sites are occupied by E1/UBAE from the sample, fewer sites are left to bind E1/UBAE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E1/UBAE concentration in each sample is interpolated from this standard curve.

Product Categories/Family for E1/UBAE elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 46389771
• NCBI GeneID: 79876
• Molecular Weight: 38,537 Da
• NCBI Official Full Name: Ubiquitin activating enzyme
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 5
• NCBI Official Symbol: UBA5
• NCBI Official Synonym Symbols: THIFP1; UBE1DC1
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 5; UFM1-activating enzyme; ubiquitin-activating enzyme 5; UBA5, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-activating enzyme E1 domain-containing protein 1
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 5
• UniProt Gene Name: UBA5
• UniProt Synonym Gene Names: UBE1DC1; Ubiquitin-activating enzyme 5
• UniProt Entry Name: UBA5_HUMAN

NCBI Description

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE1DC1: E1-like enzyme which activates UFM1 and SUMO2. Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; ATP binding

Biological Process: regulation of estrogen receptor signaling pathway