Soluble RANK Ligand Active Protein | sRANKL active protein
Recombinant Mouse Soluble RANK Ligand (158-316 a.a)
Gene Names
Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
Soluble RANK Ligand; Recombinant Mouse Soluble RANK Ligand (158-316 a.a); sRANKL (158-316) Mouse; Soluble Receptor Activator of NFkB Ligand; TNFSF11; TRANCE; TNF-related activation-induced cytokine; OPGL; ODF; Osteoclast differentiation factor; Tumor necrosis factor ligand superfamily member 11; Receptor activator of nuclear factor kappa B ligand; RANKL; Osteoprotegerin ligand; CD254 antigen; sRANKL; sOdf; Soluble RANK Ligand (158-316 a.a) Mouse Recombinant; sRANKL active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Form/Format
Sterile filtered white lyophilized (freeze-dried) powder.
sRANKL protein was lyophilized from a 0.2 um filtered solution in NaH2PO4, NaCl and EDTA.
sRANKL protein was lyophilized from a 0.2 um filtered solution in NaH2PO4, NaCl and EDTA.
Sequence
Lys158-Asp316, with an N-terminal Met, Accession # Q3TWY5
Sequence Length
316
Solubility
It is recommended to reconstitute the lyophilized sRANKL in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The ED50, as measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells, is less than 2ng/ml.
Preparation and Storage
Although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution sRANKL should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Related Product Information for sRANKL active protein
Description: sRANKL Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain (158-316 a.a) having a molecular mass of 19kDa. The sRANKL is purified by proprietary chromatographic techniques.
Introduction: RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Introduction: RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Product Categories/Family for sRANKL active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,150 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 11
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 11
NCBI Official Symbol
Tnfsf11
NCBI Official Synonym Symbols
ODF; OPGL; RANKL; Ly109l; Trance
NCBI Protein Information
tumor necrosis factor ligand superfamily member 11
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 11
UniProt Gene Name
Tnfsf11
UniProt Synonym Gene Names
Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
Uniprot Description
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed:24039232). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed:23395171, PubMed:26644563).
Research Articles on sRANKL
Similar Products
Product Notes
The sRANKL tnfsf11 (Catalog #AAA146730) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Lys158-Asp 316, with an N-terminal Met, Accession # Q3TWY5. It is sometimes possible for the material contained within the vial of "Soluble RANK Ligand, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
