super leptin antagonist Active Protein | SHLA active protein
Rat super leptin antagonist (mutant D23/L39A/D40A/F41A)
Purity
The purity of super rat leptin antagonist is greater than 98.0% as determined by:(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Synonyms
super leptin antagonist; Rat super leptin antagonist (mutant D23/L39A/D40A/F41A); Rat super-active leptin antagonist (SHLA); SHLA active protein
Host
E Coli
Purity/Purification
The purity of super rat leptin antagonist is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino of acids of super rat leptin antagonist was determined and was found to be Ala-Val-Pro-Ile-His.
Solubility
It is recommended to reconstitute the lyophilized super rat leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.
Protein Content
Protein quantization of super rat leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified super rat leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.02 ng/ug (0.2 EU/ug) of super rat leptin antagonist
Formulation
The recombinant protein was lyophilized from a concentrated (1 mg/ml) solution with 0.003mM NaHCO3.
Biological Activity
super rat leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Super rat leptin antagonist also inhibits various leptin effects in several in vitro bioassays. The inhibitory activity of super rat leptin antagonist was increased 14 to 60 fold as measured by various criteria such as binding properties to human leptin binding domain (hLBD) and in vitro and in vivo bioassays.
Preparation and Storage
Lyophilized super rat leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -20 degree C. Upon reconstitution at of super rat leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization super rat leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Related Product Information for SHLA active protein
Recombinant super rat leptin antagonist is one polypeptide chain containing 146 amino. Super rat leptin antagonist (SRLA) was initially mutated, resulting in D23L/L39A/D40A/F41A super rat leptin antagonist that was purified by proprietary chromatographic techniques. The procedure was similar to that described in in Shpilman at al., J. Biol. Chem. 286:4439-42 (2011) for mouse super leptin antagonist.
Product Categories/Family for SHLA active protein
Similar Products
Product Notes
The SHLA (Catalog #AAA400108) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino of acids of super rat leptin antagonist was determined and was found to be Ala-Val-Pr o-Ile-His. It is sometimes possible for the material contained within the vial of "super leptin antagonist, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
