Interleukin-6 Active Protein | rIL 6 active protein
Recombinant Rat Interleukin-6
Gene Names
Il6; ILg6; Ifnb2
Purity
Greater than 95.0% as determined by:Analysis by RP-HPLC
Analysis by SDS-PAGE
Synonyms
Interleukin-6; Recombinant Rat Interleukin-6; IL 6 Rat; Interleukin-6 Rat Recombinant; IFN-b2; B cell differentiation factor (BCDF); BSF-2; HPGF; HSF; MGI-2; IL-6; Interleukin HP-1; B-cell hybridoma growth factor; rIL 6 active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by:
Analysis by RP-HPLC
Analysis by SDS-PAGE
Analysis by RP-HPLC
Analysis by SDS-PAGE
Form/Format
The protein was lyophilized without any additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser
Sequence Length
211
Solubility
It is recommended to reconstitute the lyophilized Interleukin-6 in 100mM acetic acid to 1.0mg/ml and incubated for 30 minutes at room temperature to regain full activity, which can then be further diluted to other aqueous soltutions.
Protein Content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
Biological Activity
The ED50 = 0.03-0.1 ng/ml corresponding to a specific activity of 10,000,000-34,000,000IU/mg. The biological activity is determined by measuring the dose-dependent proliferation of IL-6 dependent B9 cells. A concentration range of 0.1-10.0 ng/ml is effective for most in vitro applications.
Preparation and Storage
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL6 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for rIL 6 active protein
Description: Interleukin-6 Rat Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21732 Dalton. The IL6 is purified by proprietary chromatographic techniques.
Introduction: Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
Introduction: Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
Product Categories/Family for rIL 6 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
UniProt Protein Name
Interleukin-6
Protein Family
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_RAT
NCBI Description
a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
Uniprot Description
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().
Research Articles on rIL 6
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Product Notes
The rIL 6 il6 (Catalog #AAA142265) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pr o-Thr-Ser. It is sometimes possible for the material contained within the vial of "Interleukin-6, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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