Glial-Derived Neurotrophic Factor Active Protein | rGDNF active protein
Recombinant Rat Glial-Derived Neurotrophic Factor
Gene Names
Gdnf; gndf
Purity
Greater than 98.0% as determined by HPLC analysis and by SDS-PAGE.
Synonyms
Glial-Derived Neurotrophic Factor; Recombinant Rat Glial-Derived Neurotrophic Factor; GDNF Rat; Glial-Derived Neurotrophic Factor Rat Recombinant; ATF1; ATF2; HFB1-GDNF; GDNF; rGDNF active protein
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by HPLC analysis and by SDS-PAGE.
Form/Format
GDNF was lyophilized from a sterile solution containing 1xPBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
SPDKQAALP RRENRNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCESAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDNLVY HILRKHSAKR CGCI
Sequence Length
211
Solubility
It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Recombinant rat GDNF has full biological activity when compared to standards. The ED50, determined by a cell proliferation assay using rat C6 cells, is less than 0.2ng/ml corresponding to a specific activity of more than 5,000,000IU/mg.
Preparation and Storage
Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GDNF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for rGDNF active protein
Description: Glial derived Neurotrophic Factor Rat Recombinant produced in E Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 134 amino acids and having a total molecular mass of 29.8 kDa.
Introduction: GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Introduction: GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Product Categories/Family for rGDNF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,551 Da
NCBI Official Full Name
glial cell line-derived neurotrophic factor
NCBI Official Synonym Full Names
glial cell derived neurotrophic factor
NCBI Official Symbol
Gdnf
NCBI Official Synonym Symbols
gndf
NCBI Protein Information
glial cell line-derived neurotrophic factor; ATF; Glial cell line derived neutrophic factor; astrocyte-derived trophic factor; glial cell line derived neurotrophic factor
UniProt Protein Name
Glial cell line-derived neurotrophic factor
UniProt Gene Name
Gdnf
UniProt Synonym Gene Names
ATF
UniProt Entry Name
GDNF_RAT
Similar Products
Product Notes
The rGDNF gdnf (Catalog #AAA142279) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: SPDKQAALP RRENRNRQAA An ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCESAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDNLVY HILRKHSAKR CGCI . It is sometimes possible for the material contained within the vial of "Glial-Derived Neurotrophic Factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
