Beta Defensin -4 Active Protein | rBD 4 active protein
Recombinant Rat Beta Defensin -4
Gene Names
Defb4; Defb2; Defb3
Purity
Greater than 97.0% as determined by SDS-PAGE.
Synonyms
Beta Defensin -4; Recombinant Rat Beta Defensin -4; BD 4 Rat; Beta Defensin-4 Rat Recombinant; Beta-defensin 4; BD-4; BD-2; Defensin; beta 4; RBD-2; RBD-4; Defb4; Defb2; Defb3; rBD 4 active protein
Host
E Coli
Purity/Purification
Greater than 97.0% as determined by SDS-PAGE.
Form/Format
BD-4 protein was lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K
Sequence Length
63
Solubility
It is recommended to reconstitute the lyophilized BD-4 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 5-50 ug/ml.
Preparation and Storage
Lyophilized BD-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution BD-4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Related Product Information for rBD 4 active protein
Description: BD-4 Rat Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.4kDa.The BD-4 is purified by proprietary chromatographic techniques.
Introduction: Defensins are cationic peptides with a large spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The Alpha defensins are differentiated from the Beta-defensins by the pairing of their 3 disulfide bonds. 4 human Beta-defensins have been identified to date; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.
Introduction: Defensins are cationic peptides with a large spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The Alpha defensins are differentiated from the Beta-defensins by the pairing of their 3 disulfide bonds. 4 human Beta-defensins have been identified to date; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.
Product Categories/Family for rBD 4 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
6,946 Da
NCBI Official Full Name
beta-defensin 4
NCBI Official Synonym Full Names
defensin beta 4
NCBI Official Symbol
Defb4
NCBI Official Synonym Symbols
Defb2; Defb3
NCBI Protein Information
beta-defensin 4; BD-2; BD-4; RBD-2; RBD-4; beta defensin-2; defensin beta 3
UniProt Protein Name
Beta-defensin 4
UniProt Gene Name
Defb4
UniProt Synonym Gene Names
Defb2; Defb3; BD-4
UniProt Entry Name
DEFB4_RAT
NCBI Description
cationic peptides with broad-spectrum antimicrobial activity; may mediate mucosal defenses of several organs [RGD, Feb 2006]
Uniprot Description
Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells.
Research Articles on rBD 4
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Product Notes
The rBD 4 defb4 (Catalog #AAA144437) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K. It is sometimes possible for the material contained within the vial of "Beta Defensin -4, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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