Cyclophilin A Active Protein | PPIA active protein

Human Recombinant Cyclophilin A

Cat. Num. AAA844294

• Gene Names: PPIA; CYPA; CYPH; HEL-S-69p
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Cyclophilin A; Human Recombinant Cyclophilin A; Peptidylprolyl isomerase A; PPIA; CYPA; CYPH; PPIA active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20 mM NaCl, 0.5 mM DTT, 10% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE
• Sequence Length: 105

• Applicable Applications for PPIA active protein: SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 mug of protein (determined by LAL method)
• Biological Activity: Specific activity is > 270 nmoles/min/mg
• Unit Definition: Specific activity is defined as the amount of enzyme that cleaves 1 umole of Suc-AAFP-pNA per minute at 25 degree C in Tris HCl pH 8.0 using chymotrypsin.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PPIA active protein

Background: Cyclophilin A is a multifunctional protein that is upregulated in a variety of inflammatory conditions, such as rheumatoid arthritis, autoimmune disease, and cancer. It has been classified as an immunophilin and has a variety of intracellular functions, including intracellular signaling, protein trafficking, and the regulation of other proteins activity. Besides its intracellular functions, Cyclophilin A is a secreted molecule that has a physiological and pathological role in cardiovascular diseases, making it a potential biomarker and mediator in cardiovascular diseases, such as vascular stenosis, atherosclerosis, and abdominal aortic aneurysms.

Product Categories/Family for PPIA active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  

NCBI and Uniprot Product Information

• NCBI GI #: 665821274
• NCBI GeneID: 5478
• NCBI Accession #: NP_001287910.1
• NCBI GenBank Nucleotide #: NM_001300981.1
• UniProt Accession #: P62937; P05092; Q3KQW3; Q567Q0; Q6IBU5; Q96IX3; Q9BRU4; Q9BTY9; Q9UC61; A8K220
• Molecular Weight: 20 kDa
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase A isoform 2
• NCBI Official Synonym Full Names: peptidylprolyl isomerase A (cyclophilin A)
• NCBI Official Symbol: PPIA
• NCBI Official Synonym Symbols: CYPA; CYPH; HEL-S-69p
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase A
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase A
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: PPIA
• UniProt Synonym Gene Names: CYPA; PPIase A
• UniProt Entry Name: PPIA_HUMAN

NCBI Description

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PPIA: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Interacts with HIV-1 Capsid protein. Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase. Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.

Protein type: EC 5.2.1.8; RNA-binding; Cyclophilin; Isomerase

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: extracellular space; focal adhesion; membrane; extracellular region; nucleus; cytosol

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; unfolded protein binding; virion binding; peptide binding

Biological Process: release of virus from host; platelet activation; uncoating of virus; positive regulation of viral genome replication; protein peptidyl-prolyl isomerization; protein folding; viral reproduction; RNA-dependent DNA replication; virus assembly; viral infectious cycle; platelet degranulation; positive regulation of protein secretion; entry into host cell; blood coagulation; leukocyte migration; regulation of viral genome replication