Human Cyclophilin A (CYPA) ELISA Kit | CYPA elisa kit

Human Cyclophilin A (CYPA) ELISA Kit

Cat. Num. AAA454609

• Gene Names: PPIA; CYPA; CYPH; HEL-S-69p
• Reactivity: Human
• Synonyms: Cyclophilin A (CYPA); Human Cyclophilin A (CYPA) ELISA Kit; AM2; CYPA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CYPA. No significant cross-reactivity or interference between CYPA and analogues was observed.
• Sequence Length: 105

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: <0.124ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYPA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYPA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYPA elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CYPA in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CYPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CYPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 665821274
• NCBI GeneID: 5478
• NCBI Accession #: NP_001287910.1
• NCBI GenBank Nucleotide #: NM_001300981.1
• UniProt Accession #: P62937; P05092; Q3KQW3; Q567Q0; Q6IBU5; Q96IX3; Q9BRU4; Q9BTY9; Q9UC61; A8K220
• Molecular Weight: 11,418 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase A isoform 2
• NCBI Official Synonym Full Names: peptidylprolyl isomerase A
• NCBI Official Symbol: PPIA
• NCBI Official Synonym Symbols: CYPA; CYPH; HEL-S-69p
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase A
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase A
• Protein Family: Cypemycin
• UniProt Gene Name: PPIA
• UniProt Synonym Gene Names: CYPA; PPIase A

NCBI Description

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.