Phosphoglycerate Kinase 2 Active Protein | PGK2 active protein

Recombinant Human Phosphoglycerate Kinase 2, BioActive

Cat. Num. AAA141602

• Gene Names: PGK2; PGKB; PGKPS; HEL-S-272; dJ417L20.2
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Phosphoglycerate Kinase 2; Recombinant Human Phosphoglycerate Kinase 2; BioActive; PGK2 Active; PGK2 Human Active; Phosphoglycerate Kinase 2 Human Recombinant; Phosphoglycerate kinase 2; dJ417L202; PGKB; PGKPSS; Phosphoglycerate kinase; testis specific; PGK2 active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; The PGK2 solution (0.5mg/ml) contains 20% glycerol, 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 0.1M NaCl.
• Sequence: MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM

• Biological Activity: Specific activity >500unit/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH8.0 at 37 degree C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.; Store, frozen at -20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for PGK2 active protein

PGK2, also known as Phosphoglycerate kinase 2, is a testis-specific form of phosphoglycerate kinase.Originally, it was assumed that PGK2was a pseudogene. However, nowadays it is known that this protein is a functional phosphoglycerate kinase. During the Embden-Meyerhof-Parnas pathway of glycolysis, in thelater stages of spermatogenesis, the protein catalyses the reversible conversion of 1, 3- bisphosphoglycerate to 3-phosphoglycerate.
PGK2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 437 amino acids (1-417a.a.) and having a molecular mass of 46.9kDa.
PGK2 is fused to a 20 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for PGK2 active protein

PROTEIN KINASES

NCBI and Uniprot Product Information

• NCBI GI #: 31543397
• NCBI GeneID: 5232
• NCBI Accession #: NP_620061.2
• NCBI GenBank Nucleotide #: NM_138733.4
• UniProt Accession #: P07205; Q9H107; B2R6Y8
• Molecular Weight: 44,796 Da
• NCBI Official Full Name: phosphoglycerate kinase 2
• NCBI Official Synonym Full Names: phosphoglycerate kinase 2
• NCBI Official Symbol: PGK2
• NCBI Official Synonym Symbols: PGKB; PGKPS; HEL-S-272; dJ417L20.2
• NCBI Protein Information: phosphoglycerate kinase 2; epididymis secretory protein Li 272; phosphoglycerate kinase autosomal pseudogene; phosphoglycerate kinase, testis specific
• UniProt Protein Name: Phosphoglycerate kinase 2
• Protein Family: Phosphoglycerate kinase
• UniProt Gene Name: PGK2
• UniProt Synonym Gene Names: PGKB
• UniProt Entry Name: PGK2_HUMAN

NCBI Description

This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]

Uniprot Description

PGK2: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.2.3; Kinase, other

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: cytoplasm; nucleus

Molecular Function: phosphoglycerate kinase activity; ATP binding

Biological Process: sperm motility; glycolysis; phosphorylation