Oncostatin-M Active Protein | OSM active protein

Recombinant Human Oncostatin-M

Cat. Num. AAA142108

• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Oncostatin-M; Recombinant Human Oncostatin-M; OSM Human; Oncostatin-M Human Recombinant; OSM; MGC20461; OSM active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a concentrated (1mg/ml) solution containing PBS pH-7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCRERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLERSGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQPPTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPHQALRKGVRRT RPSRKGKRLM TRGQLPR
• Sequence Length: 252

• Solubility: It is recommended to reconstitute the lyophilized Oncostatin M in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of Oncostatin as Reference.
• Biological Activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000IU/mg.
• Preparation and Storage: Lyophilized Oncostatin M although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Oncostatin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for OSM active protein

Description: Oncostatin-M Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids and having a molecular mass of 26kDa. The OSM is purified by proprietary chromatographic techniques.

Introduction: Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells.

Product Categories/Family for OSM active protein

Cytokines and Growth Factors; Growth Factors; Oncostatin-M

NCBI and Uniprot Product Information

• NCBI GI #: 10092621
• NCBI GeneID: 5008
• NCBI Accession #: NP_065391.1
• NCBI GenBank Nucleotide #: NM_020530.4
• UniProt Accession #: P13725; Q6FHP8; Q9UCP6
• Molecular Weight: 28,484 Da
• NCBI Official Full Name: oncostatin-M preproprotein
• NCBI Official Synonym Full Names: oncostatin M
• NCBI Official Symbol: OSM
• NCBI Protein Information: oncostatin-M
• UniProt Protein Name: Oncostatin-M
• Protein Family: Oncostatin
• UniProt Gene Name: OSM
• UniProt Synonym Gene Names: OSM
• UniProt Entry Name: ONCM_HUMAN

NCBI Description

Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. [provided by RefSeq, Jul 2008]

Uniprot Description

OSM: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Belongs to the LIF/OSM family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: extracellular space

Molecular Function: growth factor activity; oncostatin-M receptor binding; cytokine activity

Biological Process: negative regulation of hormone secretion; multicellular organismal development; tyrosine phosphorylation of Stat3 protein; tyrosine phosphorylation of Stat5 protein; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; cell proliferation; negative regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; behavioral response to pain; positive regulation of MAPKKK cascade; response to heat; positive regulation of acute inflammatory response; positive regulation of cell division; regulation of growth; negative regulation of meiosis; positive regulation of cell proliferation; tyrosine phosphorylation of Stat1 protein; immune response; positive regulation of transcription from RNA polymerase II promoter