Desmoglein-3 (Dsg3) Active Protein | Dsg3 active protein

Recombinant Mouse Desmoglein-3 (Dsg3), partial (Active)

Cat. Num. AAA9018401

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Desmoglein-3 (Dsg3); Recombinant Mouse Desmoglein-3 (Dsg3); partial (Active); (Desmoglein-3)(130kDa pemphigus vulgaris antigen homolog); Dsg3 active protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 50-617aa; Partial
• Sequence: EWVKFAKPCREREDNSRRNPIAKITSDFQKNQKITYRISGVGIDQPPFGIFVVDPNNGDINITAIVDREETPSFLITCRALNALGQDVERPLILTVKILDVNDNPPIFSQTIFKGEIEENSASNSLVMILNATDADEPNHMNSKIAFKIVSQEPAGMSMFLISRNTGEVRTLTSSLDREQISSYHLVVSGADNDGTGLSTQCECSIKIKDVNDNFPVLRESQYSARIEENTLNAELLRFQVTDWDEEYTDNWLAVYFFTSGNEGNWFEIETDPRTNEGILKVVKALDYEQVQSMQFSIAVRNKAEFHQSVISQYRVQSTPVTIQVIDVREGISFRPPSKTFTVQRGVSTNKLVGYILGTYQATDEDTGKAASSVRYVLGRNDGGLLVIDSKTAQIKFVKNIDRDSTFIVNKTISAEVLAIDENTGKTSTGTIYVEVPSFNENCPSVVLEKKDICTSSPSVTLSVRTLDRGKYTGPYTVSLEEQPLKLPVMWTITTLNATSALLQAQQQVSPGVYNVPVIVKDNQDGLCDTPESLTLTVCQCDDRSMCRAPIPSREPNTYGESSWRLGP

• Species: Mus musculus (Mouse)
• Endotoxin: Less than 1.0EU/ug as determined by LAL method.
• Relevance: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Dsg3 at 5ug/mL can bind Anti-DSG3 recombinant antibody , the EC50 is 2.624-3.182ug/mL.
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.; Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

SDS-Page

((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

Activity

Product Categories/Family for Dsg3 active protein

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 25452923
• NCBI GeneID: 13512
• UniProt Accession #: O35902; Q8CB02; Q8CE48
• Molecular Weight: 101,344 Da
• NCBI Official Full Name: Desmoglein-3
• UniProt Protein Name: Desmoglein-3
• Protein Family: Desmoglein
• UniProt Gene Name: Dsg3
• UniProt Entry Name: DSG3_MOUSE

Uniprot Description

DSG3: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: cell junction; desmosome; integral to membrane; integral to plasma membrane; membrane; plasma membrane; spot adherens junction

Molecular Function: calcium ion binding; metal ion binding

Biological Process: cell adhesion; homophilic cell adhesion